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A prominent and conserved role for YY1 in Xist transcriptional activation
Accumulation of the non-coding RNA Xist on one X chromosome in female cells is a hallmark of X-chromosome inactivation in eutherians. Here, we uncovered an essential function for the ubiquitous autosomal transcription factor Yin-Yang 1 (YY1) in the transcriptional activation of Xist in both human an...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172967/ https://www.ncbi.nlm.nih.gov/pubmed/25209548 http://dx.doi.org/10.1038/ncomms5878 |
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author | Makhlouf, Mélanie Ouimette, Jean-François Oldfield, Andrew Navarro, Pablo Neuillet, Damien Rougeulle, Claire |
author_facet | Makhlouf, Mélanie Ouimette, Jean-François Oldfield, Andrew Navarro, Pablo Neuillet, Damien Rougeulle, Claire |
author_sort | Makhlouf, Mélanie |
collection | PubMed |
description | Accumulation of the non-coding RNA Xist on one X chromosome in female cells is a hallmark of X-chromosome inactivation in eutherians. Here, we uncovered an essential function for the ubiquitous autosomal transcription factor Yin-Yang 1 (YY1) in the transcriptional activation of Xist in both human and mouse. We show that loss of YY1 prevents Xist up-regulation during the initiation and maintenance of X-inactivation, and that YY1 binds directly the Xist 5′ region to trigger the activity of the Xist promoter. Binding of YY1 to the Xist 5′ region prior to X-chromosome inactivation competes with the Xist repressor REX1 while DNA methylation controls mono-allelic fixation of YY1 to Xist at the onset of X-chromosome inactivation. YY1 is thus the first autosomal activating factor involved in a fundamental and conserved pathway of Xist regulation that ensures the asymmetric transcriptional up-regulation of the master regulator of X-chromosome inactivation. |
format | Online Article Text |
id | pubmed-4172967 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
record_format | MEDLINE/PubMed |
spelling | pubmed-41729672015-03-11 A prominent and conserved role for YY1 in Xist transcriptional activation Makhlouf, Mélanie Ouimette, Jean-François Oldfield, Andrew Navarro, Pablo Neuillet, Damien Rougeulle, Claire Nat Commun Article Accumulation of the non-coding RNA Xist on one X chromosome in female cells is a hallmark of X-chromosome inactivation in eutherians. Here, we uncovered an essential function for the ubiquitous autosomal transcription factor Yin-Yang 1 (YY1) in the transcriptional activation of Xist in both human and mouse. We show that loss of YY1 prevents Xist up-regulation during the initiation and maintenance of X-inactivation, and that YY1 binds directly the Xist 5′ region to trigger the activity of the Xist promoter. Binding of YY1 to the Xist 5′ region prior to X-chromosome inactivation competes with the Xist repressor REX1 while DNA methylation controls mono-allelic fixation of YY1 to Xist at the onset of X-chromosome inactivation. YY1 is thus the first autosomal activating factor involved in a fundamental and conserved pathway of Xist regulation that ensures the asymmetric transcriptional up-regulation of the master regulator of X-chromosome inactivation. 2014-09-11 /pmc/articles/PMC4172967/ /pubmed/25209548 http://dx.doi.org/10.1038/ncomms5878 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Makhlouf, Mélanie Ouimette, Jean-François Oldfield, Andrew Navarro, Pablo Neuillet, Damien Rougeulle, Claire A prominent and conserved role for YY1 in Xist transcriptional activation |
title | A prominent and conserved role for YY1 in Xist transcriptional activation |
title_full | A prominent and conserved role for YY1 in Xist transcriptional activation |
title_fullStr | A prominent and conserved role for YY1 in Xist transcriptional activation |
title_full_unstemmed | A prominent and conserved role for YY1 in Xist transcriptional activation |
title_short | A prominent and conserved role for YY1 in Xist transcriptional activation |
title_sort | prominent and conserved role for yy1 in xist transcriptional activation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172967/ https://www.ncbi.nlm.nih.gov/pubmed/25209548 http://dx.doi.org/10.1038/ncomms5878 |
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