Generation of catalytic human Ago4 identifies structural elements important for RNA cleavage

Argonaute proteins bind small RNAs and mediate cleavage of complementary target RNAs. The human Argonaute protein Ago4 is catalytically inactive, although it is highly similar to catalytic Ago2. Here, we have generated Ago2-Ago4 chimeras and analyzed their cleavage activity in vitro. We identify sev...

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Detalles Bibliográficos
Autores principales: Hauptmann, Judith, Kater, Lukas, Löffler, Patrick, Merkl, Rainer, Meister, Gunter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2014
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174435/
https://www.ncbi.nlm.nih.gov/pubmed/25114291
http://dx.doi.org/10.1261/rna.045203.114

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174435/
https://www.ncbi.nlm.nih.gov/pubmed/25114291
http://dx.doi.org/10.1261/rna.045203.114

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