Cargando…

A cluster of methylations in the domain IV of 25S rRNA is required for ribosome stability

In all three domains of life ribosomal RNAs are extensively modified at functionally important sites of the ribosome. These modifications are believed to fine-tune the ribosome structure for optimal translation. However, the precise mechanistic effect of modifications on ribosome function remains la...

Descripción completa

Detalles Bibliográficos
Autores principales: Gigova, Andriana, Duggimpudi, Sujitha, Pollex, Tim, Schaefer, Matthias, Koš, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174444/
https://www.ncbi.nlm.nih.gov/pubmed/25125595
http://dx.doi.org/10.1261/rna.043398.113
_version_ 1782336350076272640
author Gigova, Andriana
Duggimpudi, Sujitha
Pollex, Tim
Schaefer, Matthias
Koš, Martin
author_facet Gigova, Andriana
Duggimpudi, Sujitha
Pollex, Tim
Schaefer, Matthias
Koš, Martin
author_sort Gigova, Andriana
collection PubMed
description In all three domains of life ribosomal RNAs are extensively modified at functionally important sites of the ribosome. These modifications are believed to fine-tune the ribosome structure for optimal translation. However, the precise mechanistic effect of modifications on ribosome function remains largely unknown. Here we show that a cluster of methylated nucleotides in domain IV of 25S rRNA is critical for integrity of the large ribosomal subunit. We identified the elusive cytosine-5 methyltransferase for C2278 in yeast as Rcm1 and found that a combined loss of cytosine-5 methylation at C2278 and ribose methylation at G2288 caused dramatic ribosome instability, resulting in loss of 60S ribosomal subunits. Structural and biochemical analyses revealed that this instability was caused by changes in the structure of 25S rRNA and a consequent loss of multiple ribosomal proteins from the large ribosomal subunit. Our data demonstrate that individual RNA modifications can strongly affect structure of large ribonucleoprotein complexes.
format Online
Article
Text
id pubmed-4174444
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Cold Spring Harbor Laboratory Press
record_format MEDLINE/PubMed
spelling pubmed-41744442015-10-01 A cluster of methylations in the domain IV of 25S rRNA is required for ribosome stability Gigova, Andriana Duggimpudi, Sujitha Pollex, Tim Schaefer, Matthias Koš, Martin RNA Article In all three domains of life ribosomal RNAs are extensively modified at functionally important sites of the ribosome. These modifications are believed to fine-tune the ribosome structure for optimal translation. However, the precise mechanistic effect of modifications on ribosome function remains largely unknown. Here we show that a cluster of methylated nucleotides in domain IV of 25S rRNA is critical for integrity of the large ribosomal subunit. We identified the elusive cytosine-5 methyltransferase for C2278 in yeast as Rcm1 and found that a combined loss of cytosine-5 methylation at C2278 and ribose methylation at G2288 caused dramatic ribosome instability, resulting in loss of 60S ribosomal subunits. Structural and biochemical analyses revealed that this instability was caused by changes in the structure of 25S rRNA and a consequent loss of multiple ribosomal proteins from the large ribosomal subunit. Our data demonstrate that individual RNA modifications can strongly affect structure of large ribonucleoprotein complexes. Cold Spring Harbor Laboratory Press 2014-10 /pmc/articles/PMC4174444/ /pubmed/25125595 http://dx.doi.org/10.1261/rna.043398.113 Text en © 2014 Gigova et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Article
Gigova, Andriana
Duggimpudi, Sujitha
Pollex, Tim
Schaefer, Matthias
Koš, Martin
A cluster of methylations in the domain IV of 25S rRNA is required for ribosome stability
title A cluster of methylations in the domain IV of 25S rRNA is required for ribosome stability
title_full A cluster of methylations in the domain IV of 25S rRNA is required for ribosome stability
title_fullStr A cluster of methylations in the domain IV of 25S rRNA is required for ribosome stability
title_full_unstemmed A cluster of methylations in the domain IV of 25S rRNA is required for ribosome stability
title_short A cluster of methylations in the domain IV of 25S rRNA is required for ribosome stability
title_sort cluster of methylations in the domain iv of 25s rrna is required for ribosome stability
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174444/
https://www.ncbi.nlm.nih.gov/pubmed/25125595
http://dx.doi.org/10.1261/rna.043398.113
work_keys_str_mv AT gigovaandriana aclusterofmethylationsinthedomainivof25srrnaisrequiredforribosomestability
AT duggimpudisujitha aclusterofmethylationsinthedomainivof25srrnaisrequiredforribosomestability
AT pollextim aclusterofmethylationsinthedomainivof25srrnaisrequiredforribosomestability
AT schaefermatthias aclusterofmethylationsinthedomainivof25srrnaisrequiredforribosomestability
AT kosmartin aclusterofmethylationsinthedomainivof25srrnaisrequiredforribosomestability
AT gigovaandriana clusterofmethylationsinthedomainivof25srrnaisrequiredforribosomestability
AT duggimpudisujitha clusterofmethylationsinthedomainivof25srrnaisrequiredforribosomestability
AT pollextim clusterofmethylationsinthedomainivof25srrnaisrequiredforribosomestability
AT schaefermatthias clusterofmethylationsinthedomainivof25srrnaisrequiredforribosomestability
AT kosmartin clusterofmethylationsinthedomainivof25srrnaisrequiredforribosomestability