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A cluster of methylations in the domain IV of 25S rRNA is required for ribosome stability
In all three domains of life ribosomal RNAs are extensively modified at functionally important sites of the ribosome. These modifications are believed to fine-tune the ribosome structure for optimal translation. However, the precise mechanistic effect of modifications on ribosome function remains la...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174444/ https://www.ncbi.nlm.nih.gov/pubmed/25125595 http://dx.doi.org/10.1261/rna.043398.113 |
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author | Gigova, Andriana Duggimpudi, Sujitha Pollex, Tim Schaefer, Matthias Koš, Martin |
author_facet | Gigova, Andriana Duggimpudi, Sujitha Pollex, Tim Schaefer, Matthias Koš, Martin |
author_sort | Gigova, Andriana |
collection | PubMed |
description | In all three domains of life ribosomal RNAs are extensively modified at functionally important sites of the ribosome. These modifications are believed to fine-tune the ribosome structure for optimal translation. However, the precise mechanistic effect of modifications on ribosome function remains largely unknown. Here we show that a cluster of methylated nucleotides in domain IV of 25S rRNA is critical for integrity of the large ribosomal subunit. We identified the elusive cytosine-5 methyltransferase for C2278 in yeast as Rcm1 and found that a combined loss of cytosine-5 methylation at C2278 and ribose methylation at G2288 caused dramatic ribosome instability, resulting in loss of 60S ribosomal subunits. Structural and biochemical analyses revealed that this instability was caused by changes in the structure of 25S rRNA and a consequent loss of multiple ribosomal proteins from the large ribosomal subunit. Our data demonstrate that individual RNA modifications can strongly affect structure of large ribonucleoprotein complexes. |
format | Online Article Text |
id | pubmed-4174444 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-41744442015-10-01 A cluster of methylations in the domain IV of 25S rRNA is required for ribosome stability Gigova, Andriana Duggimpudi, Sujitha Pollex, Tim Schaefer, Matthias Koš, Martin RNA Article In all three domains of life ribosomal RNAs are extensively modified at functionally important sites of the ribosome. These modifications are believed to fine-tune the ribosome structure for optimal translation. However, the precise mechanistic effect of modifications on ribosome function remains largely unknown. Here we show that a cluster of methylated nucleotides in domain IV of 25S rRNA is critical for integrity of the large ribosomal subunit. We identified the elusive cytosine-5 methyltransferase for C2278 in yeast as Rcm1 and found that a combined loss of cytosine-5 methylation at C2278 and ribose methylation at G2288 caused dramatic ribosome instability, resulting in loss of 60S ribosomal subunits. Structural and biochemical analyses revealed that this instability was caused by changes in the structure of 25S rRNA and a consequent loss of multiple ribosomal proteins from the large ribosomal subunit. Our data demonstrate that individual RNA modifications can strongly affect structure of large ribonucleoprotein complexes. Cold Spring Harbor Laboratory Press 2014-10 /pmc/articles/PMC4174444/ /pubmed/25125595 http://dx.doi.org/10.1261/rna.043398.113 Text en © 2014 Gigova et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Article Gigova, Andriana Duggimpudi, Sujitha Pollex, Tim Schaefer, Matthias Koš, Martin A cluster of methylations in the domain IV of 25S rRNA is required for ribosome stability |
title | A cluster of methylations in the domain IV of 25S rRNA is required for ribosome stability |
title_full | A cluster of methylations in the domain IV of 25S rRNA is required for ribosome stability |
title_fullStr | A cluster of methylations in the domain IV of 25S rRNA is required for ribosome stability |
title_full_unstemmed | A cluster of methylations in the domain IV of 25S rRNA is required for ribosome stability |
title_short | A cluster of methylations in the domain IV of 25S rRNA is required for ribosome stability |
title_sort | cluster of methylations in the domain iv of 25s rrna is required for ribosome stability |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174444/ https://www.ncbi.nlm.nih.gov/pubmed/25125595 http://dx.doi.org/10.1261/rna.043398.113 |
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