Direct elicitation of template concentration from quantification cycle (C(q)) distributions in digital PCR

Digital PCR (dPCR) exploits limiting dilution of a template into an array of PCR reactions. From this array the number of reactions that contain at least one (as opposed to zero) initial template is determined, allowing inferring the original template concentration. Here we present a novel protocol...

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Detalles Bibliográficos
Autores principales: Mojtahedi, Mitra, Fouquier d'Hérouël, Aymeric, Huang, Sui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4176345/
https://www.ncbi.nlm.nih.gov/pubmed/25104023
http://dx.doi.org/10.1093/nar/gku603

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4176345/
https://www.ncbi.nlm.nih.gov/pubmed/25104023
http://dx.doi.org/10.1093/nar/gku603

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