Secretion of a foreign protein from budding yeasts is enhanced by cotranslational translocation and by suppression of vacuolar targeting

BACKGROUND: Budding yeasts are often used to secrete foreign proteins, but the efficiency is variable. To identify roadblocks in the yeast secretory pathway, we used a monomeric superfolder GFP (msGFP) as a visual tracer in Saccharomyces cerevisiae and Pichia pastoris. RESULTS: One roadblock for msG...

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Detalles Bibliográficos
Autores principales: Fitzgerald, Ivy, Glick, Benjamin S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4176846/
https://www.ncbi.nlm.nih.gov/pubmed/25164324
http://dx.doi.org/10.1186/s12934-014-0125-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4176846/
https://www.ncbi.nlm.nih.gov/pubmed/25164324
http://dx.doi.org/10.1186/s12934-014-0125-0

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