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Enhanced expression of a 35 kDa fragment of inter-alpha-trypsin inhibitor H4 in sera of healthy pregnant women and patients with hydatidiform mole

BACKGROUND: Accumulated data from previous studies appear to suggest a link between the overexpression of a 35 kDa fragment of serum inter-alpha-trypsin inhibitor H4 (ITIH4) with cancers that are associated with up-regulated levels of oestrogens. The truncated fragment was postulated to be a product...

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Autores principales: Mohamed, Emida, Jayapalan, Jaime Jacqueline, Abdul-Rahman, Puteri Shafinaz, Omar, Siti Zawiah, Hashim, Onn Haji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4177572/
https://www.ncbi.nlm.nih.gov/pubmed/24252421
http://dx.doi.org/10.1186/2050-7771-1-19
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author Mohamed, Emida
Jayapalan, Jaime Jacqueline
Abdul-Rahman, Puteri Shafinaz
Omar, Siti Zawiah
Hashim, Onn Haji
author_facet Mohamed, Emida
Jayapalan, Jaime Jacqueline
Abdul-Rahman, Puteri Shafinaz
Omar, Siti Zawiah
Hashim, Onn Haji
author_sort Mohamed, Emida
collection PubMed
description BACKGROUND: Accumulated data from previous studies appear to suggest a link between the overexpression of a 35 kDa fragment of serum inter-alpha-trypsin inhibitor H4 (ITIH4) with cancers that are associated with up-regulated levels of oestrogens. The truncated fragment was postulated to be a product of oestrogen-induced action of kallikrein on native ITIH4. The present lectin-based proteomic analyses were performed to assess the specificity of the 35 kDa fragment of ITIH4 as a potential cancer biomarker and determine whether it was also overexpressed in the sera of cancer-negative pregnant women who are known to have high levels of plasma oestrogens. RESULTS: Our results demonstrated that the 35 kDa fragment of ITIH4 was overexpressed in healthy pregnant women and patients with hydatidiform mole, relative to the controls. The serum oestradiol levels of both groups of pregnant subjects were also confirmed to be higher than those of the control women who were not pregnant. CONCLUSIONS: Overexpression of the 35 kDa fragment of ITIH4 was not restrictive to patients with cancers but also occurred in women who were pregnant and those diagnosed with hydatidiform mole. Our data implicate the limitation of the 35 kDa ITIH4 fragment as a cancer biomarker and its correlation with serum oestrogen levels.
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spelling pubmed-41775722014-09-29 Enhanced expression of a 35 kDa fragment of inter-alpha-trypsin inhibitor H4 in sera of healthy pregnant women and patients with hydatidiform mole Mohamed, Emida Jayapalan, Jaime Jacqueline Abdul-Rahman, Puteri Shafinaz Omar, Siti Zawiah Hashim, Onn Haji Biomark Res Research BACKGROUND: Accumulated data from previous studies appear to suggest a link between the overexpression of a 35 kDa fragment of serum inter-alpha-trypsin inhibitor H4 (ITIH4) with cancers that are associated with up-regulated levels of oestrogens. The truncated fragment was postulated to be a product of oestrogen-induced action of kallikrein on native ITIH4. The present lectin-based proteomic analyses were performed to assess the specificity of the 35 kDa fragment of ITIH4 as a potential cancer biomarker and determine whether it was also overexpressed in the sera of cancer-negative pregnant women who are known to have high levels of plasma oestrogens. RESULTS: Our results demonstrated that the 35 kDa fragment of ITIH4 was overexpressed in healthy pregnant women and patients with hydatidiform mole, relative to the controls. The serum oestradiol levels of both groups of pregnant subjects were also confirmed to be higher than those of the control women who were not pregnant. CONCLUSIONS: Overexpression of the 35 kDa fragment of ITIH4 was not restrictive to patients with cancers but also occurred in women who were pregnant and those diagnosed with hydatidiform mole. Our data implicate the limitation of the 35 kDa ITIH4 fragment as a cancer biomarker and its correlation with serum oestrogen levels. BioMed Central 2013-05-15 /pmc/articles/PMC4177572/ /pubmed/24252421 http://dx.doi.org/10.1186/2050-7771-1-19 Text en Copyright © 2013 Mohamed et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Mohamed, Emida
Jayapalan, Jaime Jacqueline
Abdul-Rahman, Puteri Shafinaz
Omar, Siti Zawiah
Hashim, Onn Haji
Enhanced expression of a 35 kDa fragment of inter-alpha-trypsin inhibitor H4 in sera of healthy pregnant women and patients with hydatidiform mole
title Enhanced expression of a 35 kDa fragment of inter-alpha-trypsin inhibitor H4 in sera of healthy pregnant women and patients with hydatidiform mole
title_full Enhanced expression of a 35 kDa fragment of inter-alpha-trypsin inhibitor H4 in sera of healthy pregnant women and patients with hydatidiform mole
title_fullStr Enhanced expression of a 35 kDa fragment of inter-alpha-trypsin inhibitor H4 in sera of healthy pregnant women and patients with hydatidiform mole
title_full_unstemmed Enhanced expression of a 35 kDa fragment of inter-alpha-trypsin inhibitor H4 in sera of healthy pregnant women and patients with hydatidiform mole
title_short Enhanced expression of a 35 kDa fragment of inter-alpha-trypsin inhibitor H4 in sera of healthy pregnant women and patients with hydatidiform mole
title_sort enhanced expression of a 35 kda fragment of inter-alpha-trypsin inhibitor h4 in sera of healthy pregnant women and patients with hydatidiform mole
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4177572/
https://www.ncbi.nlm.nih.gov/pubmed/24252421
http://dx.doi.org/10.1186/2050-7771-1-19
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