A Single Amino Acid Substitution in the Novel H7N9 Influenza A Virus NS1 Protein Increases CPSF30 Binding and Virulence

Although an effective interferon antagonist in human and avian cells, the novel H7N9 influenza virus NS1 protein is defective at inhibiting CPSF30. An I106M substitution in H7N9 NS1 can restore CPSF30 binding together with the ability to block host gene expression. Furthermore, a recombinant virus e...

Descripción completa

Detalles Bibliográficos
Autores principales: Ayllon, Juan, Domingues, Patricia, Rajsbaum, Ricardo, Miorin, Lisa, Schmolke, Mirco, Hale, Benjamin G., García-Sastre, Adolfo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2014
Materias:
Pathogenesis and Immunity
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178744/
https://www.ncbi.nlm.nih.gov/pubmed/25078692
http://dx.doi.org/10.1128/JVI.01567-14
_version_ 1782336979628720128
author Ayllon, Juan
Domingues, Patricia
Rajsbaum, Ricardo
Miorin, Lisa
Schmolke, Mirco
Hale, Benjamin G.
García-Sastre, Adolfo
author_facet Ayllon, Juan
Domingues, Patricia
Rajsbaum, Ricardo
Miorin, Lisa
Schmolke, Mirco
Hale, Benjamin G.
García-Sastre, Adolfo
author_sort Ayllon, Juan
collection PubMed
description Although an effective interferon antagonist in human and avian cells, the novel H7N9 influenza virus NS1 protein is defective at inhibiting CPSF30. An I106M substitution in H7N9 NS1 can restore CPSF30 binding together with the ability to block host gene expression. Furthermore, a recombinant virus expressing H7N9 NS1-I106M replicates to higher titers in vivo, and is subtly more virulent, than the parental virus. Natural polymorphisms in H7N9 NS1 that enhance CPSF30 binding may be cause for concern.
format Online
Article
Text
id pubmed-4178744
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher American Society for Microbiology
record_format MEDLINE/PubMed
spelling pubmed-41787442015-04-01 A Single Amino Acid Substitution in the Novel H7N9 Influenza A Virus NS1 Protein Increases CPSF30 Binding and Virulence Ayllon, Juan Domingues, Patricia Rajsbaum, Ricardo Miorin, Lisa Schmolke, Mirco Hale, Benjamin G. García-Sastre, Adolfo J Virol Pathogenesis and Immunity Although an effective interferon antagonist in human and avian cells, the novel H7N9 influenza virus NS1 protein is defective at inhibiting CPSF30. An I106M substitution in H7N9 NS1 can restore CPSF30 binding together with the ability to block host gene expression. Furthermore, a recombinant virus expressing H7N9 NS1-I106M replicates to higher titers in vivo, and is subtly more virulent, than the parental virus. Natural polymorphisms in H7N9 NS1 that enhance CPSF30 binding may be cause for concern. American Society for Microbiology 2014-10 /pmc/articles/PMC4178744/ /pubmed/25078692 http://dx.doi.org/10.1128/JVI.01567-14 Text en Copyright © 2014 Ayllon et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license (http://creativecommons.org/licenses/by/3.0/) .
spellingShingle Pathogenesis and Immunity
Ayllon, Juan
Domingues, Patricia
Rajsbaum, Ricardo
Miorin, Lisa
Schmolke, Mirco
Hale, Benjamin G.
García-Sastre, Adolfo
A Single Amino Acid Substitution in the Novel H7N9 Influenza A Virus NS1 Protein Increases CPSF30 Binding and Virulence
title A Single Amino Acid Substitution in the Novel H7N9 Influenza A Virus NS1 Protein Increases CPSF30 Binding and Virulence
title_full A Single Amino Acid Substitution in the Novel H7N9 Influenza A Virus NS1 Protein Increases CPSF30 Binding and Virulence
title_fullStr A Single Amino Acid Substitution in the Novel H7N9 Influenza A Virus NS1 Protein Increases CPSF30 Binding and Virulence
title_full_unstemmed A Single Amino Acid Substitution in the Novel H7N9 Influenza A Virus NS1 Protein Increases CPSF30 Binding and Virulence
title_short A Single Amino Acid Substitution in the Novel H7N9 Influenza A Virus NS1 Protein Increases CPSF30 Binding and Virulence
title_sort single amino acid substitution in the novel h7n9 influenza a virus ns1 protein increases cpsf30 binding and virulence
topic Pathogenesis and Immunity
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178744/
https://www.ncbi.nlm.nih.gov/pubmed/25078692
http://dx.doi.org/10.1128/JVI.01567-14
work_keys_str_mv AT ayllonjuan asingleaminoacidsubstitutioninthenovelh7n9influenzaavirusns1proteinincreasescpsf30bindingandvirulence
AT dominguespatricia asingleaminoacidsubstitutioninthenovelh7n9influenzaavirusns1proteinincreasescpsf30bindingandvirulence
AT rajsbaumricardo asingleaminoacidsubstitutioninthenovelh7n9influenzaavirusns1proteinincreasescpsf30bindingandvirulence
AT miorinlisa asingleaminoacidsubstitutioninthenovelh7n9influenzaavirusns1proteinincreasescpsf30bindingandvirulence
AT schmolkemirco asingleaminoacidsubstitutioninthenovelh7n9influenzaavirusns1proteinincreasescpsf30bindingandvirulence
AT halebenjaming asingleaminoacidsubstitutioninthenovelh7n9influenzaavirusns1proteinincreasescpsf30bindingandvirulence
AT garciasastreadolfo asingleaminoacidsubstitutioninthenovelh7n9influenzaavirusns1proteinincreasescpsf30bindingandvirulence
AT ayllonjuan singleaminoacidsubstitutioninthenovelh7n9influenzaavirusns1proteinincreasescpsf30bindingandvirulence
AT dominguespatricia singleaminoacidsubstitutioninthenovelh7n9influenzaavirusns1proteinincreasescpsf30bindingandvirulence
AT rajsbaumricardo singleaminoacidsubstitutioninthenovelh7n9influenzaavirusns1proteinincreasescpsf30bindingandvirulence
AT miorinlisa singleaminoacidsubstitutioninthenovelh7n9influenzaavirusns1proteinincreasescpsf30bindingandvirulence
AT schmolkemirco singleaminoacidsubstitutioninthenovelh7n9influenzaavirusns1proteinincreasescpsf30bindingandvirulence
AT halebenjaming singleaminoacidsubstitutioninthenovelh7n9influenzaavirusns1proteinincreasescpsf30bindingandvirulence
AT garciasastreadolfo singleaminoacidsubstitutioninthenovelh7n9influenzaavirusns1proteinincreasescpsf30bindingandvirulence

Ejemplares similares