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Oxidized LDL lipids increase β-amyloid production by SH-SY5Y cells through glutathione depletion and lipid raft formation

Elevated total cholesterol in midlife has been associated with increased risk of dementia in later life. We have previously shown that low-density lipoprotein (LDL) is more oxidized in the plasma of dementia patients, although total cholesterol levels are not different from those of age-matched cont...

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Autores principales: Dias, Irundika H.K., Mistry, Jayna, Fell, Shaun, Reis, Ana, Spickett, Corinne M., Polidori, Maria C., Lip, Gregory Y.H., Griffiths, Helen R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4180009/
https://www.ncbi.nlm.nih.gov/pubmed/25048970
http://dx.doi.org/10.1016/j.freeradbiomed.2014.07.012
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author Dias, Irundika H.K.
Mistry, Jayna
Fell, Shaun
Reis, Ana
Spickett, Corinne M.
Polidori, Maria C.
Lip, Gregory Y.H.
Griffiths, Helen R.
author_facet Dias, Irundika H.K.
Mistry, Jayna
Fell, Shaun
Reis, Ana
Spickett, Corinne M.
Polidori, Maria C.
Lip, Gregory Y.H.
Griffiths, Helen R.
author_sort Dias, Irundika H.K.
collection PubMed
description Elevated total cholesterol in midlife has been associated with increased risk of dementia in later life. We have previously shown that low-density lipoprotein (LDL) is more oxidized in the plasma of dementia patients, although total cholesterol levels are not different from those of age-matched controls. β-Amyloid (Aβ) peptide, which accumulates in Alzheimer disease (AD), arises from the initial cleavage of amyloid precursor protein by β-secretase-1 (BACE1). BACE1 activity is regulated by membrane lipids and raft formation. Given the evidence for altered lipid metabolism in AD, we have investigated a mechanism for enhanced Aβ production by SH-SY5Y neuronal-like cells exposed to oxidized LDL (oxLDL). The viability of SH-SY5Y cells exposed to 4 μg oxLDL and 25 µM 27-hydroxycholesterol (27OH-C) was decreased significantly. Lipids, but not proteins, extracted from oxLDL were more cytotoxic than oxLDL. In parallel, the ratio of reduced glutathione (GSH) to oxidized glutathione was decreased at sublethal concentrations of lipids extracted from native and oxLDL. GSH loss was associated with an increase in acid sphingomyelinase (ASMase) activity and lipid raft formation, which could be inhibited by the ASMase inhibitor desipramine. 27OH-C and total lipids from LDL and oxLDL independently increased Aβ production by SH-SY5Y cells, and Aβ accumulation could be inhibited by desipramine and by N-acetylcysteine. These data suggest a mechanism whereby oxLDL lipids and 27OH-C can drive Aβ production by GSH depletion, ASMase-driven membrane remodeling, and BACE1 activation in neuronal cells.
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spelling pubmed-41800092014-10-02 Oxidized LDL lipids increase β-amyloid production by SH-SY5Y cells through glutathione depletion and lipid raft formation Dias, Irundika H.K. Mistry, Jayna Fell, Shaun Reis, Ana Spickett, Corinne M. Polidori, Maria C. Lip, Gregory Y.H. Griffiths, Helen R. Free Radic Biol Med Original Contribution Elevated total cholesterol in midlife has been associated with increased risk of dementia in later life. We have previously shown that low-density lipoprotein (LDL) is more oxidized in the plasma of dementia patients, although total cholesterol levels are not different from those of age-matched controls. β-Amyloid (Aβ) peptide, which accumulates in Alzheimer disease (AD), arises from the initial cleavage of amyloid precursor protein by β-secretase-1 (BACE1). BACE1 activity is regulated by membrane lipids and raft formation. Given the evidence for altered lipid metabolism in AD, we have investigated a mechanism for enhanced Aβ production by SH-SY5Y neuronal-like cells exposed to oxidized LDL (oxLDL). The viability of SH-SY5Y cells exposed to 4 μg oxLDL and 25 µM 27-hydroxycholesterol (27OH-C) was decreased significantly. Lipids, but not proteins, extracted from oxLDL were more cytotoxic than oxLDL. In parallel, the ratio of reduced glutathione (GSH) to oxidized glutathione was decreased at sublethal concentrations of lipids extracted from native and oxLDL. GSH loss was associated with an increase in acid sphingomyelinase (ASMase) activity and lipid raft formation, which could be inhibited by the ASMase inhibitor desipramine. 27OH-C and total lipids from LDL and oxLDL independently increased Aβ production by SH-SY5Y cells, and Aβ accumulation could be inhibited by desipramine and by N-acetylcysteine. These data suggest a mechanism whereby oxLDL lipids and 27OH-C can drive Aβ production by GSH depletion, ASMase-driven membrane remodeling, and BACE1 activation in neuronal cells. Elsevier Science 2014-10 /pmc/articles/PMC4180009/ /pubmed/25048970 http://dx.doi.org/10.1016/j.freeradbiomed.2014.07.012 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Original Contribution
Dias, Irundika H.K.
Mistry, Jayna
Fell, Shaun
Reis, Ana
Spickett, Corinne M.
Polidori, Maria C.
Lip, Gregory Y.H.
Griffiths, Helen R.
Oxidized LDL lipids increase β-amyloid production by SH-SY5Y cells through glutathione depletion and lipid raft formation
title Oxidized LDL lipids increase β-amyloid production by SH-SY5Y cells through glutathione depletion and lipid raft formation
title_full Oxidized LDL lipids increase β-amyloid production by SH-SY5Y cells through glutathione depletion and lipid raft formation
title_fullStr Oxidized LDL lipids increase β-amyloid production by SH-SY5Y cells through glutathione depletion and lipid raft formation
title_full_unstemmed Oxidized LDL lipids increase β-amyloid production by SH-SY5Y cells through glutathione depletion and lipid raft formation
title_short Oxidized LDL lipids increase β-amyloid production by SH-SY5Y cells through glutathione depletion and lipid raft formation
title_sort oxidized ldl lipids increase β-amyloid production by sh-sy5y cells through glutathione depletion and lipid raft formation
topic Original Contribution
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4180009/
https://www.ncbi.nlm.nih.gov/pubmed/25048970
http://dx.doi.org/10.1016/j.freeradbiomed.2014.07.012
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