Systematic evaluation of quantotypic peptides for targeted analysis of the human kinome

In targeted proteomics it is critical that peptides are not only proteotypic, but also accurately represent the level of the protein (quantotypic). Numerous approaches are used to identify proteotypic peptides, but quantotypic properties are rarely assessed. Here, we show that measuring ratios of pr...

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Detalles Bibliográficos
Autores principales: Worboys, Jonathan D., Sinclair, John, Yuan, Yinyin, Jørgensen, Claus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4180722/
https://www.ncbi.nlm.nih.gov/pubmed/25152083
http://dx.doi.org/10.1038/nmeth.3072
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author Worboys, Jonathan D.
Sinclair, John
Yuan, Yinyin
Jørgensen, Claus
author_facet Worboys, Jonathan D.
Sinclair, John
Yuan, Yinyin
Jørgensen, Claus
author_sort Worboys, Jonathan D.
collection PubMed
description In targeted proteomics it is critical that peptides are not only proteotypic, but also accurately represent the level of the protein (quantotypic). Numerous approaches are used to identify proteotypic peptides, but quantotypic properties are rarely assessed. Here, we show that measuring ratios of proteotypic peptides across biological samples can be used to empirically identify peptides with good quantotypic properties, and use this to identify quantotypic peptides for 21% of the human kinome.
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spelling pubmed-41807222015-04-01 Systematic evaluation of quantotypic peptides for targeted analysis of the human kinome Worboys, Jonathan D. Sinclair, John Yuan, Yinyin Jørgensen, Claus Nat Methods Article In targeted proteomics it is critical that peptides are not only proteotypic, but also accurately represent the level of the protein (quantotypic). Numerous approaches are used to identify proteotypic peptides, but quantotypic properties are rarely assessed. Here, we show that measuring ratios of proteotypic peptides across biological samples can be used to empirically identify peptides with good quantotypic properties, and use this to identify quantotypic peptides for 21% of the human kinome. 2014-08-24 2014-10 /pmc/articles/PMC4180722/ /pubmed/25152083 http://dx.doi.org/10.1038/nmeth.3072 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Worboys, Jonathan D.
Sinclair, John
Yuan, Yinyin
Jørgensen, Claus
Systematic evaluation of quantotypic peptides for targeted analysis of the human kinome
title Systematic evaluation of quantotypic peptides for targeted analysis of the human kinome
title_full Systematic evaluation of quantotypic peptides for targeted analysis of the human kinome
title_fullStr Systematic evaluation of quantotypic peptides for targeted analysis of the human kinome
title_full_unstemmed Systematic evaluation of quantotypic peptides for targeted analysis of the human kinome
title_short Systematic evaluation of quantotypic peptides for targeted analysis of the human kinome
title_sort systematic evaluation of quantotypic peptides for targeted analysis of the human kinome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4180722/
https://www.ncbi.nlm.nih.gov/pubmed/25152083
http://dx.doi.org/10.1038/nmeth.3072
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