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Human primary mixed brain cultures: preparation, differentiation, characterization and application to neuroscience research

BACKGROUND: Culturing primary cortical neurons is an essential neuroscience technique. However, most cultures are derived from rodent brains and standard protocols for human brain cultures are sparse. Herein, we describe preparation, maintenance and major characteristics of a primary human mixed bra...

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Detalles Bibliográficos
Autores principales: Ray, Balmiki, Chopra, Nipun, Long, Justin M, Lahiri, Debomoy K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4181361/
https://www.ncbi.nlm.nih.gov/pubmed/25223359
http://dx.doi.org/10.1186/s13041-014-0063-0