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Human primary mixed brain cultures: preparation, differentiation, characterization and application to neuroscience research
BACKGROUND: Culturing primary cortical neurons is an essential neuroscience technique. However, most cultures are derived from rodent brains and standard protocols for human brain cultures are sparse. Herein, we describe preparation, maintenance and major characteristics of a primary human mixed bra...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4181361/ https://www.ncbi.nlm.nih.gov/pubmed/25223359 http://dx.doi.org/10.1186/s13041-014-0063-0 |