Cargando…

Human primary mixed brain cultures: preparation, differentiation, characterization and application to neuroscience research

BACKGROUND: Culturing primary cortical neurons is an essential neuroscience technique. However, most cultures are derived from rodent brains and standard protocols for human brain cultures are sparse. Herein, we describe preparation, maintenance and major characteristics of a primary human mixed bra...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ray, Balmiki, Chopra, Nipun, Long, Justin M, Lahiri, Debomoy K
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2014
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4181361/ https://www.ncbi.nlm.nih.gov/pubmed/25223359 http://dx.doi.org/10.1186/s13041-014-0063-0

Ejemplares similares

Repeated electromagnetic field stimulation lowers amyloid-β peptide levels in primary human mixed brain tissue cultures
por: Perez, Felipe P., et al.
Publicado: (2021)

Increased Secreted Amyloid Precursor Protein-α (sAPPα) in Severe Autism: Proposal of a Specific, Anabolic Pathway and Putative Biomarker
por: Ray, Balmiki, et al.
Publicado: (2011)

Rivastigmine Lowers Aβ and Increases sAPPα Levels, Which Parallel Elevated Synaptic Markers and Metabolic Activity in Degenerating Primary Rat Neurons
por: Bailey, Jason A., et al.
Publicado: (2011)

Finding novel distinctions between the sAPPα-mediated anabolic biochemical pathways in Autism Spectrum Disorder and Fragile X Syndrome plasma and brain tissue
por: Ray, Balmiki, et al.
Publicado: (2016)

Novel Nuclear Factor-KappaB Targeting Peptide Suppresses β-Amyloid Induced Inflammatory and Apoptotic Responses in Neuronal Cells
por: Srinivasan, Mythily, et al.
Publicado: (2016)

Lithium alters expression of RNAs in a type-specific manner in differentiated human neuroblastoma neuronal cultures, including specific genes involved in Alzheimer’s disease
por: Maloney, Bryan, et al.
Publicado: (2019)

Autism as early neurodevelopmental disorder: evidence for an sAPPα-mediated anabolic pathway
por: Lahiri, Debomoy K., et al.
Publicado: (2013)

Rivastigmine modifies the α-secretase pathway and potentially early Alzheimer’s disease
por: Ray, Balmiki, et al.
Publicado: (2020)

Correction: Rivastigmine modifies the α-secretase pathway and potentially early Alzheimer’s disease
por: Ray, Balmiki, et al.
Publicado: (2020)

Human microRNA (miR-20b-5p) modulates Alzheimer’s disease pathways and neuronal function, and a specific polymorphism close to the MIR20B gene influences Alzheimer’s biomarkers
por: Wang, Ruizhi, et al.
Publicado: (2022)

Proteomic sample preparation for blast wound characterization
por: Chromy, Brett A, et al.
Publicado: (2014)

Characterization of extracellular matrix macromolecules in primary cultures of equine keratinocytes
por: Visser, Michelle B, et al.
Publicado: (2010)

Expression and testing in plants of ArcLight, a genetically–encoded voltage indicator used in neuroscience research
por: Matzke, Antonius J.M., et al.
Publicado: (2015)

Mixed methods and survey research in family medicine and community health
por: Creswell, John W, et al.
Publicado: (2019)

Organizational culture change in U.S. hospitals: a mixed methods longitudinal intervention study
por: Curry, Leslie A, et al.
Publicado: (2015)

Novel upregulation of amyloid-β precursor protein (APP) by microRNA-346 via targeting of APP mRNA 5′-untranslated region: Implications in Alzheimer’s disease
por: Long, Justin M., et al.
Publicado: (2018)

Basics of statistics for primary care research
por: Guetterman, Timothy C
Publicado: (2019)

Fully moderated t-statistic in linear modeling of mixed effects for differential expression analysis
por: Yu, Lianbo, et al.
Publicado: (2019)

Functional characterization of a competitive peptide antagonist of p65 in human macrophage-like cells suggests therapeutic potential for chronic inflammation
por: Srinivasan, Mythily, et al.
Publicado: (2014)

Validating a multi-locus metabarcoding approach for characterizing mixed-pollen samples
por: Wizenberg, Sydney B., et al.
Publicado: (2023)

Using mixed methods in health research
por: Tariq, Shema, et al.
Publicado: (2013)

Getting started in primary care research: choosing among six practical research approaches
por: Fetters, Michael D
Publicado: (2019)

MicroRNA-298 reduces levels of human amyloid-β precursor protein (APP), β-site APP-converting enzyme 1 (BACE1) and specific tau protein moieties
por: Chopra, Nipun, et al.
Publicado: (2020)

Establishment and characterization of a differentiated epithelial cell culture model derived from the porcine cervix uteri
por: Miessen, Katrin, et al.
Publicado: (2012)

Series: Practical guidance to qualitative research. Part 6: Longitudinal qualitative and mixed-methods approaches for longitudinal and complex health themes in primary care research
por: Korstjens, Irene, et al.
Publicado: (2022)

Exendin-4 Ameliorates Motor Neuron Degeneration in Cellular and Animal Models of Amyotrophic Lateral Sclerosis
por: Li, Yazhou, et al.
Publicado: (2012)

Electroporation-based methods for in vivo, whole mount and primary culture analysis of zebrafish brain development
por: Hendricks, Michael, et al.
Publicado: (2007)

High-efficiency transfection of cultured primary motor neurons to study protein localization, trafficking, and function
por: Fallini, Claudia, et al.
Publicado: (2010)

Semistructured interviewing in primary care research: a balance of relationship and rigour
por: DeJonckheere, Melissa, et al.
Publicado: (2019)

Culture, neuroscience, and law
por: Salvato, Gerardo, et al.
Publicado: (2014)

Effects of microRNA-298 on APP and BACE1 translation differ according to cell type and 3′-UTR variation
por: Wang, Ruizhi, et al.
Publicado: (2022)

Patient and public involvement in primary care research - an example of ensuring its sustainability
por: Jinks, Clare, et al.
Publicado: (2016)

Patients-people-place: developing a framework for researching organizational culture during health service redesign and change
por: Gale, Nicola K, et al.
Publicado: (2014)

An adaptable chromosome preparation methodology for use in invertebrate research organisms
por: Guo, Longhua, et al.
Publicado: (2018)

Machine learning analysis of microbial flow cytometry data from nanoparticles, antibiotics and carbon sources perturbed anaerobic microbiomes
por: Dhoble, Abhishek S., et al.
Publicado: (2018)

Analysis of cell identity, morphology, apoptosis and mitotic activity in a primary neural cell culture system in Drosophila
por: Moraru, Manuela M, et al.
Publicado: (2012)

Improved CRISPR/Cas9 gene editing in primary human myoblasts using low confluency cultures on Matrigel
por: Goullée, Hayley, et al.
Publicado: (2021)

Conducting health policy analysis in primary care research: turning clinical ideas into action
por: Engelman, Alina, et al.
Publicado: (2019)

A general approach to mixed effects modeling of residual variances in generalized linear mixed models
por: Kizilkaya, Kadir, et al.
Publicado: (2005)

Mixed methods in pre-hospital research: understanding complex clinical problems
por: Whitley, Gregory Adam, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_rfmjvmoi4r0nd0v42jp43pdiso