A Suitable Streptomycin-Resistant Mutant for Constructing Unmarked In-Frame Gene Deletions Using rpsL as a Counter-Selection Marker

The streptomycin counter-selection system is a useful tool for constructing unmarked in-frame gene deletions, which is a fundamental approach to study bacteria and their pathogenicity at the molecular level. A prerequisite for this system is acquiring a streptomycin-resistant strain due to rpsL muta...

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Detalles Bibliográficos
Autores principales: Tsai, Yu-Kuo, Liou, Ci-Hong, Lin, Jung-Chung, Ma, Ling, Fung, Chang-Phone, Chang, Feng-Yee, Siu, L. Kristopher
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4182516/
https://www.ncbi.nlm.nih.gov/pubmed/25268958
http://dx.doi.org/10.1371/journal.pone.0109258

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4182516/
https://www.ncbi.nlm.nih.gov/pubmed/25268958
http://dx.doi.org/10.1371/journal.pone.0109258

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