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Considerations for accurate gene expression measurement by reverse transcription quantitative PCR when analysing clinical samples

Reverse transcription quantitative PCR is an established, simple and effective method for RNA measurement. However, technical standardisation challenges combined with frequent insufficient experimental detail render replication of many published findings challenging. Consequently, without adequate c...

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Detalles Bibliográficos
Autores principales: Sanders, Rebecca, Mason, Deborah J., Foy, Carole A., Huggett, Jim F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4182594/
https://www.ncbi.nlm.nih.gov/pubmed/24858468
http://dx.doi.org/10.1007/s00216-014-7857-x