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Considerations for accurate gene expression measurement by reverse transcription quantitative PCR when analysing clinical samples
Reverse transcription quantitative PCR is an established, simple and effective method for RNA measurement. However, technical standardisation challenges combined with frequent insufficient experimental detail render replication of many published findings challenging. Consequently, without adequate c...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer Berlin Heidelberg
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4182594/ https://www.ncbi.nlm.nih.gov/pubmed/24858468 http://dx.doi.org/10.1007/s00216-014-7857-x |