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Engineering an Enhanced, Thermostable, Monomeric Bacterial Luciferase Gene As a Reporter in Plant Protoplasts

The application of the luxCDABE operon of the bioluminescent bacterium Photorhabdus luminescens as a reporter has been published for bacteria, yeast and mammalian cells. We report here the optimization of fused luxAB (the bacterial luciferase heterodimeric enzyme) expression, quantum yield and its a...

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Detalles Bibliográficos
Autores principales: Cui, Boyu, Zhang, Lifeng, Song, Yunhong, Wei, Jinsong, Li, Changfu, Wang, Tietao, Wang, Yao, Zhao, Tianyong, Shen, Xihui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4182741/
https://www.ncbi.nlm.nih.gov/pubmed/25271765
http://dx.doi.org/10.1371/journal.pone.0107885