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Hybridization Capture Using Short PCR Products Enriches Small Genomes by Capturing Flanking Sequences (CapFlank)

Solution hybridization capture methods utilize biotinylated oligonucleotides as baits to enrich homologous sequences from next generation sequencing (NGS) libraries. Coupled with NGS, the method generates kilo to gigabases of high confidence consensus targeted sequence. However, in many experiments,...

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Detalles Bibliográficos
Autores principales: Tsangaras, Kyriakos, Wales, Nathan, Sicheritz-Pontén, Thomas, Rasmussen, Simon, Michaux, Johan, Ishida, Yasuko, Morand, Serge, Kampmann, Marie-Louise, Gilbert, M. Thomas P., Greenwood, Alex D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4183570/
https://www.ncbi.nlm.nih.gov/pubmed/25275614
http://dx.doi.org/10.1371/journal.pone.0109101