Human Serotonin Receptor 5-HT(1A) Preferentially Segregates to the Liquid Disordered Phase in Synthetic Lipid Bilayers

[Image: see text] We demonstrate successful incorporation of the G protein coupled receptor 5-HT(1A) into giant unilamellar vesicles using an agarose rehydration method. With direct observation using fluorescence techniques, we report preferential segregation of 5-HT(1A) into the cholesterol-poor li...

Descripción completa

Detalles Bibliográficos
Autores principales: Gutierrez, M. Gertrude, Malmstadt, Noah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4183657/
https://www.ncbi.nlm.nih.gov/pubmed/25211019
http://dx.doi.org/10.1021/ja507221m
_version_ 1782337736053620736
author Gutierrez, M. Gertrude
Malmstadt, Noah
author_facet Gutierrez, M. Gertrude
Malmstadt, Noah
author_sort Gutierrez, M. Gertrude
collection PubMed
description [Image: see text] We demonstrate successful incorporation of the G protein coupled receptor 5-HT(1A) into giant unilamellar vesicles using an agarose rehydration method. With direct observation using fluorescence techniques, we report preferential segregation of 5-HT(1A) into the cholesterol-poor liquid disordered phase of the membrane, contradicting previous reports of lipid raft segregation. Furthermore, altering the concentration of cholesterol and sphingomyelin in ternary mixtures does not alter 5-HT(1A) segregation into the liquid disordered phase.
format Online
Article
Text
id pubmed-4183657
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-41836572015-09-11 Human Serotonin Receptor 5-HT(1A) Preferentially Segregates to the Liquid Disordered Phase in Synthetic Lipid Bilayers Gutierrez, M. Gertrude Malmstadt, Noah J Am Chem Soc [Image: see text] We demonstrate successful incorporation of the G protein coupled receptor 5-HT(1A) into giant unilamellar vesicles using an agarose rehydration method. With direct observation using fluorescence techniques, we report preferential segregation of 5-HT(1A) into the cholesterol-poor liquid disordered phase of the membrane, contradicting previous reports of lipid raft segregation. Furthermore, altering the concentration of cholesterol and sphingomyelin in ternary mixtures does not alter 5-HT(1A) segregation into the liquid disordered phase. American Chemical Society 2014-09-11 2014-10-01 /pmc/articles/PMC4183657/ /pubmed/25211019 http://dx.doi.org/10.1021/ja507221m Text en Copyright © 2014 American Chemical Society Terms of Use (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html)
spellingShingle Gutierrez, M. Gertrude
Malmstadt, Noah
Human Serotonin Receptor 5-HT(1A) Preferentially Segregates to the Liquid Disordered Phase in Synthetic Lipid Bilayers
title Human Serotonin Receptor 5-HT(1A) Preferentially Segregates to the Liquid Disordered Phase in Synthetic Lipid Bilayers
title_full Human Serotonin Receptor 5-HT(1A) Preferentially Segregates to the Liquid Disordered Phase in Synthetic Lipid Bilayers
title_fullStr Human Serotonin Receptor 5-HT(1A) Preferentially Segregates to the Liquid Disordered Phase in Synthetic Lipid Bilayers
title_full_unstemmed Human Serotonin Receptor 5-HT(1A) Preferentially Segregates to the Liquid Disordered Phase in Synthetic Lipid Bilayers
title_short Human Serotonin Receptor 5-HT(1A) Preferentially Segregates to the Liquid Disordered Phase in Synthetic Lipid Bilayers
title_sort human serotonin receptor 5-ht(1a) preferentially segregates to the liquid disordered phase in synthetic lipid bilayers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4183657/
https://www.ncbi.nlm.nih.gov/pubmed/25211019
http://dx.doi.org/10.1021/ja507221m
work_keys_str_mv AT gutierrezmgertrude humanserotoninreceptor5ht1apreferentiallysegregatestotheliquiddisorderedphaseinsyntheticlipidbilayers
AT malmstadtnoah humanserotoninreceptor5ht1apreferentiallysegregatestotheliquiddisorderedphaseinsyntheticlipidbilayers

Ejemplares similares