Base-cleavable microarrays for the characterization of DNA and RNA oligonucleotides synthesized in situ by photolithography

Assessing synthesis efficiency, errors, failed deprotections, and chemical and enzymatic degradation of oligonucleotides on microarrays is essential for improving existing in situ synthesis methods, and for the development of new chemistries. We describe the use of LC-MS to analyse DNA and RNA oligo...

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Detalles Bibliográficos
Autores principales: Lietard, Jory, Kretschy, Nicole, Sack, Matej, Wahba, Alexander S., Somoza, Mark M., Damha, Masad J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2014
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4183992/
https://www.ncbi.nlm.nih.gov/pubmed/25213224
http://dx.doi.org/10.1039/c4cc05771f
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author Lietard, Jory
Kretschy, Nicole
Sack, Matej
Wahba, Alexander S.
Somoza, Mark M.
Damha, Masad J.
author_facet Lietard, Jory
Kretschy, Nicole
Sack, Matej
Wahba, Alexander S.
Somoza, Mark M.
Damha, Masad J.
author_sort Lietard, Jory
collection PubMed
description Assessing synthesis efficiency, errors, failed deprotections, and chemical and enzymatic degradation of oligonucleotides on microarrays is essential for improving existing in situ synthesis methods, and for the development of new chemistries. We describe the use of LC-MS to analyse DNA and RNA oligonucleotides deprotected and cleaved under basic conditions from microarrays fabricated using light-directed in situ chemistry. The data yield essential information on array quality and sequence identity.
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spelling pubmed-41839922014-10-17 Base-cleavable microarrays for the characterization of DNA and RNA oligonucleotides synthesized in situ by photolithography Lietard, Jory Kretschy, Nicole Sack, Matej Wahba, Alexander S. Somoza, Mark M. Damha, Masad J. Chem Commun (Camb) Chemistry Assessing synthesis efficiency, errors, failed deprotections, and chemical and enzymatic degradation of oligonucleotides on microarrays is essential for improving existing in situ synthesis methods, and for the development of new chemistries. We describe the use of LC-MS to analyse DNA and RNA oligonucleotides deprotected and cleaved under basic conditions from microarrays fabricated using light-directed in situ chemistry. The data yield essential information on array quality and sequence identity. Royal Society of Chemistry 2014-11-04 2014-09-12 /pmc/articles/PMC4183992/ /pubmed/25213224 http://dx.doi.org/10.1039/c4cc05771f Text en This journal is © The Royal Society of Chemistry 2014 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Lietard, Jory
Kretschy, Nicole
Sack, Matej
Wahba, Alexander S.
Somoza, Mark M.
Damha, Masad J.
Base-cleavable microarrays for the characterization of DNA and RNA oligonucleotides synthesized in situ by photolithography
title Base-cleavable microarrays for the characterization of DNA and RNA oligonucleotides synthesized in situ by photolithography
title_full Base-cleavable microarrays for the characterization of DNA and RNA oligonucleotides synthesized in situ by photolithography
title_fullStr Base-cleavable microarrays for the characterization of DNA and RNA oligonucleotides synthesized in situ by photolithography
title_full_unstemmed Base-cleavable microarrays for the characterization of DNA and RNA oligonucleotides synthesized in situ by photolithography
title_short Base-cleavable microarrays for the characterization of DNA and RNA oligonucleotides synthesized in situ by photolithography
title_sort base-cleavable microarrays for the characterization of dna and rna oligonucleotides synthesized in situ by photolithography
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4183992/
https://www.ncbi.nlm.nih.gov/pubmed/25213224
http://dx.doi.org/10.1039/c4cc05771f
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