Selective Microbial Genomic DNA Isolation Using Restriction Endonucleases

To improve the metagenomic analysis of complex microbiomes, we have repurposed restriction endonucleases as methyl specific DNA binding proteins. As an example, we use DpnI immobilized on magnetic beads. The ten minute extraction technique allows specific binding of genomes containing the DpnI G(m6)...

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Detalles Bibliográficos
Autores principales: Barnes, Helen E., Liu, Guohong, Weston, Christopher Q., King, Paula, Pham, Long K., Waltz, Shannon, Helzer, Kimberly T., Day, Laura, Sphar, Dan, Yamamoto, Robert T., Forsyth, R. Allyn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4184833/
https://www.ncbi.nlm.nih.gov/pubmed/25279840
http://dx.doi.org/10.1371/journal.pone.0109061

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4184833/
https://www.ncbi.nlm.nih.gov/pubmed/25279840
http://dx.doi.org/10.1371/journal.pone.0109061

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