Cargando…

Selective Microbial Genomic DNA Isolation Using Restriction Endonucleases

To improve the metagenomic analysis of complex microbiomes, we have repurposed restriction endonucleases as methyl specific DNA binding proteins. As an example, we use DpnI immobilized on magnetic beads. The ten minute extraction technique allows specific binding of genomes containing the DpnI G(m6)...

Descripción completa

Detalles Bibliográficos
Autores principales:	Barnes, Helen E., Liu, Guohong, Weston, Christopher Q., King, Paula, Pham, Long K., Waltz, Shannon, Helzer, Kimberly T., Day, Laura, Sphar, Dan, Yamamoto, Robert T., Forsyth, R. Allyn
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4184833/ https://www.ncbi.nlm.nih.gov/pubmed/25279840 http://dx.doi.org/10.1371/journal.pone.0109061

Ejemplares similares

Epigenetic Segregation of Microbial Genomes from Complex Samples Using Restriction Endonucleases HpaII and McrB
por: Liu, Guohong, et al.
Publicado: (2016)

Correction: Longitudinal Metagenomic Analysis of Hospital Air Identifies Clinically Relevant Microbes
por: King, Paula, et al.
Publicado: (2016)

Longitudinal Metagenomic Analysis of Hospital Air Identifies Clinically Relevant Microbes
por: King, Paula, et al.
Publicado: (2016)

Restriction glycosylases: involvement of endonuclease activities in the restriction process
por: Zhang, Yingbiao, et al.
Publicado: (2017)

Solitary restriction endonucleases in prokaryotic genomes
por: Ershova, Anna S., et al.
Publicado: (2012)

Massively parallel characterization of restriction endonucleases
por: Kamps-Hughes, Nick, et al.
Publicado: (2013)

Selection of restriction endonucleases using artificial cells
por: Zheng, Yu, et al.
Publicado: (2007)

Cofactor Requirement of HpyAV Restriction Endonuclease
por: Chan, Siu-Hong, et al.
Publicado: (2010)

RNA aptamer inhibitors of a restriction endonuclease
por: Mondragón, Estefanía, et al.
Publicado: (2015)

Creating highly specific nucleases by fusion of active restriction endonucleases and catalytically inactive homing endonucleases
por: Fonfara, Ines, et al.
Publicado: (2012)

Using Restriction Endonuclease, Protection, Selection, and Amplification to Identify Preferred DNA-Binding Sequences of Microbial Transcription Factors
por: Barrows, John K., et al.
Publicado: (2023)

Type I restriction endonucleases are true catalytic enzymes
por: Bianco, Piero R., et al.
Publicado: (2009)

Characterization of the 5-hydroxymethylcytosine-specific DNA restriction endonucleases
por: Borgaro, Janine G., et al.
Publicado: (2013)

Catalytic domain of restriction endonuclease BmrI as a cleavage module for engineering endonucleases with novel substrate specificities
por: Chan, Siu-hong, et al.
Publicado: (2007)

Analyzing the forces binding a restriction endonuclease to DNA using a synthetic nanopore
por: Dorvel, B., et al.
Publicado: (2009)

Dynamics and consequences of DNA looping by the FokI restriction endonuclease
por: Catto, Lucy E., et al.
Publicado: (2008)

Dynamics and consequences of DNA looping by the FokI restriction endonuclease
por: Catto, Lucy E., et al.
Publicado: (2008)

Protein assembly and DNA looping by the FokI restriction endonuclease
por: Catto, Lucy E., et al.
Publicado: (2006)

Frequent occurrence of recognition Site-like sequences in the restriction endonucleases
por: Biro, Jan C, et al.
Publicado: (2004)

Simple and Cost-Effective Restriction Endonuclease Analysis of Human Adenoviruses
por: Adhikary, Arun Kumar, et al.
Publicado: (2014)

Type II restriction endonucleases—a historical perspective and more
por: Pingoud, Alfred, et al.
Publicado: (2014)

Increasing cleavage specificity and activity of restriction endonuclease KpnI
por: Vasu, Kommireddy, et al.
Publicado: (2013)

Editorial: NAR Surveys the Past, Present and Future of Restriction Endonucleases
por: Dynan, William, et al.
Publicado: (2014)

On the role of steric clashes in methylation control of restriction endonuclease activity
por: Mierzejewska, Karolina, et al.
Publicado: (2016)

Endonuclease Specificity and Sequence Dependence of Type IIS Restriction Enzymes
por: Lundin, Sverker, et al.
Publicado: (2015)

Type II restriction endonucleases — a historical perspective and more
por: Pingoud, Alfred, et al.
Publicado: (2016)

A physical map of human Alu repeats cleavage by restriction endonucleases
por: Abdurashitov, Murat A, et al.
Publicado: (2008)

Restriction Endonucleases that Bridge and Excise Two Recognition Sites from DNA
por: Marshall, Jacqueline J.T., et al.
Publicado: (2007)

Developing a programmed restriction endonuclease for highly specific DNA cleavage
por: Eisenschmidt, Kristin, et al.
Publicado: (2005)

A homology model of restriction endonuclease SfiI in complex with DNA
por: Chmiel, Agnieszka A, et al.
Publicado: (2005)

Concerted action at eight phosphodiester bonds by the BcgI restriction endonuclease
por: Marshall, Jacqueline J. T., et al.
Publicado: (2011)

Rational engineering of sequence specificity in R.MwoI restriction endonuclease
por: Skowronek, Krzysztof, et al.
Publicado: (2012)

A unique family of Mrr-like modification-dependent restriction endonucleases
por: Zheng, Yu, et al.
Publicado: (2010)

Alteration of Sequence Specificity of the Type IIS Restriction Endonuclease BtsI
por: Guan, Shengxi, et al.
Publicado: (2010)

The Type II restriction endonuclease MvaI has dual specificity
por: Stier, Ildikó, et al.
Publicado: (2010)

Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases
por: Macíčková-Cahová, Hana, et al.
Publicado: (2009)

Structure and mutagenesis of the DNA modification-dependent restriction endonuclease AspBHI
por: Horton, John R., et al.
Publicado: (2014)

Natural tuning of restriction endonuclease synthesis by cluster of rare arginine codons
por: Mruk, Iwona, et al.
Publicado: (2019)

A protein architecture guided screen for modification dependent restriction endonucleases
por: Lutz, Thomas, et al.
Publicado: (2019)

Structures, activity and mechanism of the Type IIS restriction endonuclease PaqCI
por: Kennedy, Madison A, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_35ij8281ci9rc3ut2s5363n3lo