Efficient generation of knock-in transgenic zebrafish carrying reporter/driver genes by CRISPR/Cas9-mediated genome engineering

The type II bacterial CRISPR/Cas9 system is rapidly becoming popular for genome-engineering due to its simplicity, flexibility, and high efficiency. Recently, targeted knock-in of a long DNA fragment via homology-independent DNA repair has been achieved in zebrafish using CRISPR/Cas9 system. This ra...

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Detalles Bibliográficos
Autores principales: Kimura, Yukiko, Hisano, Yu, Kawahara, Atsuo, Higashijima, Shin-ichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189020/
https://www.ncbi.nlm.nih.gov/pubmed/25293390
http://dx.doi.org/10.1038/srep06545

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189020/
https://www.ncbi.nlm.nih.gov/pubmed/25293390
http://dx.doi.org/10.1038/srep06545

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