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Molecular cloning of Reteplase and its expression in E. coli using tac promoter

BACKGROUND AND AIMS: This study aimed to clone and express the reteplase cDNA, a thrombolytic agent used for the treatment of acute myocardial infarction and stroke, in E. coli, utilizing tac promoter for its expression. MATERIALS AND METHODS: Reteplase cDNA was amplified by polymerase chain reactio...

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Detalles Bibliográficos
Autores principales: Aghaabdollahian, Safieh, Rabbani, Mohammad, Ghaedi, Kamran, Sadeghi, Hamid Mir Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189210/
https://www.ncbi.nlm.nih.gov/pubmed/25298959
http://dx.doi.org/10.4103/2277-9175.140622