Cargando…

Molecular cloning of Reteplase and its expression in E. coli using tac promoter

BACKGROUND AND AIMS: This study aimed to clone and express the reteplase cDNA, a thrombolytic agent used for the treatment of acute myocardial infarction and stroke, in E. coli, utilizing tac promoter for its expression. MATERIALS AND METHODS: Reteplase cDNA was amplified by polymerase chain reactio...

Descripción completa

Detalles Bibliográficos
Autores principales:	Aghaabdollahian, Safieh, Rabbani, Mohammad, Ghaedi, Kamran, Sadeghi, Hamid Mir Mohammad
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Medknow Publications & Media Pvt Ltd 2014
Materias:	Brief Report
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189210/ https://www.ncbi.nlm.nih.gov/pubmed/25298959 http://dx.doi.org/10.4103/2277-9175.140622

Ejemplares similares

Cloning and Expression of Functional Reteplase in Escherichia coli TOP10
por: Khodabakhsh, Fatemeh, et al.
Publicado: (2013)

Optimization of the Expression of Reteplase in Escherichia coli TOP10 Using Arabinose Promoter
por: Shafiee, Fatemeh, et al.
Publicado: (2015)

Optimization of the expression of reteplase in Escherichia coli
por: Sadeghi, H. Mir Mohammad, et al.
Publicado: (2011)

Optimization of Fermentation Conditions for Reteplase Expression by Escherichia coli Using Response Surface Methodology
por: Zare, Hamze, et al.
Publicado: (2019)

Reteplase: Structure, Function, and Production
por: Mohammadi, Elmira, et al.
Publicado: (2019)

Improvement of Soluble Production of Reteplase in Escherichia coli by Optimization of Chemical Chaperones in Lysis Buffer
por: Fazeli, Behnaz, et al.
Publicado: (2019)

Effect of buffer additives on solubilization and refolding of reteplase inclusion bodies
por: Esmaili, Iman, et al.
Publicado: (2018)

Expression of the recombinant plasminogen activator (reteplase) by a non-lytic insect cell expression system
por: Aflakiyan, S., et al.
Publicado: (2013)

Molecular cloning of gluconobacter oxydans DSM 2003 xylitol dehydrogenase gene
por: Sadeghi, H. Mir Mohammad, et al.
Publicado: (2011)

Improving the solubility, activity, and stability of reteplase using in silico design of new variants
por: Ghaheh, Hooria Seyedhosseini, et al.
Publicado: (2019)

Investigation of Supercharging as A Strategy to Enhance the Solubility and Plasminogen Cleavage Activity of Reteplase
por: Seyedhosseini Ghaheh, Hooria, et al.
Publicado: (2020)

Ionizing Radiation Promotes Epithelial-Mesenchymal Transition Phenotype and Stem Cell Marker in The Lung adenocarcinoma: In Vitro and Bioinformatic Studies
por: Raei, Mehdi, et al.
Publicado: (2022)

An unusual form of TAC–TAC sine autonomic phenomena
por: Pugach, Neil Lewis
Publicado: (2008)

Comparison of Three Escherichia coli Strains in Recombinant Production of Reteplase
por: Fathi-Roudsari, Mehrnoosh, et al.
Publicado: (2016)

Cloning and Expression of Randomly Mutated Bacillus subtilis α-Amylase Genes in HB101
por: Rabbani, Mohammed, et al.
Publicado: (2011)

Thrombolysis with reteplase in acute pulmonary embolism
por: Nishanth, K.R., et al.
Publicado: (2019)

Association of KCNJ11 (E23K) gene polymorphism with susceptibility to type 2 diabetes in Iranian patients
por: Rastegari, Ali, et al.
Publicado: (2015)

Cloning, Expression, and in vitro Functional Activity Assay of phiC31 Integrase cDNA in Escherichia coli
por: Sekhavati, Mohammad Hadi, et al.
Publicado: (2013)

Successful treatment of prosthetic pulmonary valve thrombosis with reteplase: a case report
por: Bagheri, Ramin Khameneh, et al.
Publicado: (2018)

Molecular Cloning and Identification of the Transcriptional Regulatory Domain of the Goat Neurokinin B Gene TAC3
por: SUETOMI, Yuta, et al.
Publicado: (2013)

FTIR Investigation of Secondary Structure of Reteplase Inclusion Bodies Produced in Escherichia coli in Terms of Urea Concentration
por: Naeimipour, Sajjad, et al.
Publicado: (2020)

Design of an improved multiplex PCR method for diagnosis of enterohaemoraghic E.coli and enteropathogic E.coli pathotypes
por: Soleimani, Mohammad, et al.
Publicado: (2012)

Rational Development of A Polycistronic Plasmid with A CpG-Free Bacterial Backbone as A Potential Tool for Direct Reprogramming
por: Dormiani, Kianoush, et al.
Publicado: (2017)

Microvascular obstruction after successful fibrinolytic therapy in acute myocardial infarction. Comparison of reteplase vs reteplase+abciximab: A cardiovascular magnetic resonance study
por: ZONI, ANTONELLO, et al.
Publicado: (2006)

Inactivation of aprE Gene in Bacillus subtilis 168 by Homologus Recombination
por: Rabbani, Mohammed, et al.
Publicado: (2014)

Role of Benzodiazepines in the management of agitation due to inappropriate use of naltrexone
por: Sabzghabaee, Ali Mohammad, et al.
Publicado: (2012)

Successful Treatment of Massive Pulmonary Thromboembolism with Reteplase: Case Series
por: Ghobadi, Hassan, et al.
Publicado: (2018)

Stuck aortic valve treated by reteplase in a Bentall patient
por: Tanyeli, Ömer, et al.
Publicado: (2015)

Response Surface Methodology to Optimize the Expression Efficiency of Recombinant Reteplase
por: Farzaneh, Farhad, et al.
Publicado: (2023)

Tic-tac sans tac-tic
Publicado: (1999)

Study of the Role of siRNA Mediated Promoter Methylation in DNMT3B Knockdown and Alteration of Promoter Methylation of CDH1, GSTP1 Genes in MDA-MB -453 Cell Line
por: Naghitorabi, Mojgan, et al.
Publicado: (2017)

Identification and molecular cloning of glutamate decarboxylase gene from Lactobacillus casei
por: Tavakoli, Yasaman, et al.
Publicado: (2015)

Insights Into The Effects of Amino Acid Substitutions on The Stability of Reteplase Structure: A Molecular Dynamics Simulation Study
por: Haji-Allahverdipoor, Kaveh, et al.
Publicado: (2023)

Identification and cloning of putative water clarification genes of Moringa peregrina (Forssk.) Fiori in E. coli Xl(1) blue cells
por: Ghodsi, Reihaneh, et al.
Publicado: (2014)

Cloning and Expression of Poly 3-Hydroxybutyrate Operon Into Escherichia coli
por: Jari, Maryam, et al.
Publicado: (2015)

Asn(12) and Asn(278): Critical Residues for In Vitro Biological Activity of Reteplase
por: Mandi, Naganath, et al.
Publicado: (2010)

Evaluation of efficacy and safety of Reteplase and Alteplase in the treatment of hyper-acute cerebral infarction
por: Lin, Zhi-Jian, et al.
Publicado: (2018)

Comparison of urokinase and reteplase thrombolytic treatment in patients with high-risk pulmonary embolism
por: Zhang, Yi, et al.
Publicado: (2019)

The effect of mutation on neurotoxicity reduction of new chimeric reteplase, a computational study
por: pour, Pardis Mohammadi, et al.
Publicado: (2023)

Isolation, cloning, and expression of E. coli BirA gene for biotinylation applications
por: Etemadzadeh, Mohammad Hossein, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_gcdm62r630isjmgv56e1c7in2j