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Direct Correlation of DNA Binding and Single Protein Domain Motion via Dual Illumination Fluorescence Microscopy

[Image: see text] We report a dual illumination, single-molecule imaging strategy to dissect directly and in real-time the correlation between nanometer-scale domain motion of a DNA repair protein and its interaction with individual DNA substrates. The strategy was applied to XPD, an FeS cluster-con...

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Detalles Bibliográficos
Autores principales: Ghoneim, Mohamed, Spies, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189620/
https://www.ncbi.nlm.nih.gov/pubmed/25204359
http://dx.doi.org/10.1021/nl502890g