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Anti-Heparanase Aptamers as Potential Diagnostic and Therapeutic Agents for Oral Cancer
Heparanase is an endoglycosidase enzyme present in activated leucocytes, mast cells, placental tissue, neutrophils and macrophages, and is involved in tumour metastasis and tissue invasion. It presents a potential target for cancer therapies and various molecules have been developed in an attempt to...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189786/ https://www.ncbi.nlm.nih.gov/pubmed/25295847 http://dx.doi.org/10.1371/journal.pone.0096846 |
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author | Simmons, Suzanne C. Jämsä, Hannaleena Silva, Dilson Cortez, Celia M. McKenzie, Edward A. Bitu, Carolina C. Salo, Sirpa Nurmenniemi, Sini Nyberg, Pia Risteli, Juha deAlmeida, Carlos E. B. Brenchley, Paul E. C. Salo, Tuula Missailidis, Sotiris |
author_facet | Simmons, Suzanne C. Jämsä, Hannaleena Silva, Dilson Cortez, Celia M. McKenzie, Edward A. Bitu, Carolina C. Salo, Sirpa Nurmenniemi, Sini Nyberg, Pia Risteli, Juha deAlmeida, Carlos E. B. Brenchley, Paul E. C. Salo, Tuula Missailidis, Sotiris |
author_sort | Simmons, Suzanne C. |
collection | PubMed |
description | Heparanase is an endoglycosidase enzyme present in activated leucocytes, mast cells, placental tissue, neutrophils and macrophages, and is involved in tumour metastasis and tissue invasion. It presents a potential target for cancer therapies and various molecules have been developed in an attempt to inhibit the enzymatic action of heparanase. In an attempt to develop a novel therapeutic with an associated diagnostic assay, we have previously described high affinity aptamers selected against heparanase. In this work, we demonstrated that these anti-heparanase aptamers are capable of inhibiting tissue invasion of tumour cells associated with oral cancer and verified that such inhibition is due to inhibition of the enzyme and not due to other potentially cytotoxic effects of the aptamers. Furthermore, we have identified a short 30 bases aptamer as a potential candidate for further studies, as this showed a higher ability to inhibit tissue invasion than its longer counterpart, as well as a reduced potential for complex formation with other non-specific serum proteins. Finally, the aptamer was found to be stable and therefore suitable for use in human models, as it showed no degradation in the presence of human serum, making it a potential candidate for both diagnostic and therapeutic use. |
format | Online Article Text |
id | pubmed-4189786 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41897862014-10-10 Anti-Heparanase Aptamers as Potential Diagnostic and Therapeutic Agents for Oral Cancer Simmons, Suzanne C. Jämsä, Hannaleena Silva, Dilson Cortez, Celia M. McKenzie, Edward A. Bitu, Carolina C. Salo, Sirpa Nurmenniemi, Sini Nyberg, Pia Risteli, Juha deAlmeida, Carlos E. B. Brenchley, Paul E. C. Salo, Tuula Missailidis, Sotiris PLoS One Research Article Heparanase is an endoglycosidase enzyme present in activated leucocytes, mast cells, placental tissue, neutrophils and macrophages, and is involved in tumour metastasis and tissue invasion. It presents a potential target for cancer therapies and various molecules have been developed in an attempt to inhibit the enzymatic action of heparanase. In an attempt to develop a novel therapeutic with an associated diagnostic assay, we have previously described high affinity aptamers selected against heparanase. In this work, we demonstrated that these anti-heparanase aptamers are capable of inhibiting tissue invasion of tumour cells associated with oral cancer and verified that such inhibition is due to inhibition of the enzyme and not due to other potentially cytotoxic effects of the aptamers. Furthermore, we have identified a short 30 bases aptamer as a potential candidate for further studies, as this showed a higher ability to inhibit tissue invasion than its longer counterpart, as well as a reduced potential for complex formation with other non-specific serum proteins. Finally, the aptamer was found to be stable and therefore suitable for use in human models, as it showed no degradation in the presence of human serum, making it a potential candidate for both diagnostic and therapeutic use. Public Library of Science 2014-10-08 /pmc/articles/PMC4189786/ /pubmed/25295847 http://dx.doi.org/10.1371/journal.pone.0096846 Text en © 2014 Simmons et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Simmons, Suzanne C. Jämsä, Hannaleena Silva, Dilson Cortez, Celia M. McKenzie, Edward A. Bitu, Carolina C. Salo, Sirpa Nurmenniemi, Sini Nyberg, Pia Risteli, Juha deAlmeida, Carlos E. B. Brenchley, Paul E. C. Salo, Tuula Missailidis, Sotiris Anti-Heparanase Aptamers as Potential Diagnostic and Therapeutic Agents for Oral Cancer |
title | Anti-Heparanase Aptamers as Potential Diagnostic and Therapeutic Agents for Oral Cancer |
title_full | Anti-Heparanase Aptamers as Potential Diagnostic and Therapeutic Agents for Oral Cancer |
title_fullStr | Anti-Heparanase Aptamers as Potential Diagnostic and Therapeutic Agents for Oral Cancer |
title_full_unstemmed | Anti-Heparanase Aptamers as Potential Diagnostic and Therapeutic Agents for Oral Cancer |
title_short | Anti-Heparanase Aptamers as Potential Diagnostic and Therapeutic Agents for Oral Cancer |
title_sort | anti-heparanase aptamers as potential diagnostic and therapeutic agents for oral cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189786/ https://www.ncbi.nlm.nih.gov/pubmed/25295847 http://dx.doi.org/10.1371/journal.pone.0096846 |
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