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Structure of cohesin subcomplex pinpoints direct shugoshin–Wapl antagonism in centromeric cohesion

Orderly termination of sister-chromatid cohesion during mitosis is critical for accurate chromosome segregation. During prophase, mitotic kinases phosphorylate cohesin and its protector sororin, triggering Wapl-dependent cohesin release from chromosome arms. The shugoshin (Sgo1)–PP2A complex protect...

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Detalles Bibliográficos
Autores principales: Hara, Kodai, Zheng, Ge, Qu, Qianhui, Liu, Hong, Ouyang, Zhuqing, Chen, Zhe, Tomchick, Diana R., Yu, Hongtao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4190070/
https://www.ncbi.nlm.nih.gov/pubmed/25173175
http://dx.doi.org/10.1038/nsmb.2880
Descripción
Sumario:Orderly termination of sister-chromatid cohesion during mitosis is critical for accurate chromosome segregation. During prophase, mitotic kinases phosphorylate cohesin and its protector sororin, triggering Wapl-dependent cohesin release from chromosome arms. The shugoshin (Sgo1)–PP2A complex protects centromeric cohesin until its cleavage by separase at anaphase onset. Here, we report the crystal structure of a human cohesin subcomplex comprising SA2 and Scc1. Multiple HEAT repeats of SA2 form a dragon-shaped structure. Scc1 makes extensive contacts with SA2, with one binding hotspot. Sgo1 and Wapl compete for binding to a conserved site on SA2–Scc1. Mutations of SA2 residues at this site that disrupt Wapl binding bypass Sgo1 requirement in cohesion protection. Thus, besides recruiting PP2A to dephosphorylate cohesin and sororin, Sgo1 physically shields cohesin from Wapl. This unexpected, direct antagonism between Sgo1 and Wapl augments centromeric cohesion protection.