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Evaluation of pre-induction temperature, cell growth at induction and IPTG concentration on the expression of a leptospiral protein in E. coli using shaking flasks and microbioreactor

BACKGROUND: Leptospirosis is a zoonose that is increasingly endemic in built-up areas, especially where there are communities living in precarious housing with poor or non-existent sanitation infrastructure. Leptospirosis can kill, for its symptoms are easily confused with those of other diseases. A...

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Detalles Bibliográficos
Autores principales: Larentis, Ariane Leites, Nicolau, Júlia Fabiana Monteiro Quintal, Esteves, Gabriela dos Santos, Vareschini, Daniel Tait, de Almeida, Fernanda Vicente Ribeiro, dos Reis, Mitermayer Galvão, Galler, Ricardo, Medeiros, Marco Alberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4190419/
https://www.ncbi.nlm.nih.gov/pubmed/25252618
http://dx.doi.org/10.1186/1756-0500-7-671