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Evaluation of pre-induction temperature, cell growth at induction and IPTG concentration on the expression of a leptospiral protein in E. coli using shaking flasks and microbioreactor
BACKGROUND: Leptospirosis is a zoonose that is increasingly endemic in built-up areas, especially where there are communities living in precarious housing with poor or non-existent sanitation infrastructure. Leptospirosis can kill, for its symptoms are easily confused with those of other diseases. A...
Autores principales: | Larentis, Ariane Leites, Nicolau, Júlia Fabiana Monteiro Quintal, Esteves, Gabriela dos Santos, Vareschini, Daniel Tait, de Almeida, Fernanda Vicente Ribeiro, dos Reis, Mitermayer Galvão, Galler, Ricardo, Medeiros, Marco Alberto |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4190419/ https://www.ncbi.nlm.nih.gov/pubmed/25252618 http://dx.doi.org/10.1186/1756-0500-7-671 |
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