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Affinity Purification of O-Acetylserine(thiol)lyase from Chlorella sorokiniana by Recombinant Proteins from Arabidopsis thaliana

In the unicellular green alga Chlorella sorokiniana (211/8 k), the protein O-acetylserine(thiol)lyase (OASTL), representing the key-enzyme in the biosynthetic cysteine pathway, was isolated and purified to apparent homogeneity. The purification was carried out in cells grown in the presence of all n...

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Detalles Bibliográficos
Autores principales: Salbitani, Giovanna, Wirtz, Markus, Hell, Rüdiger, Carfagna, Simona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4192684/
https://www.ncbi.nlm.nih.gov/pubmed/25093930
http://dx.doi.org/10.3390/metabo4030629