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IVT-seq reveals extreme bias in RNA sequencing

BACKGROUND: RNA-seq is a powerful technique for identifying and quantifying transcription and splicing events, both known and novel. However, given its recent development and the proliferation of library construction methods, understanding the bias it introduces is incomplete but critical to realizi...

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Detalles Bibliográficos
Autores principales: Lahens, Nicholas F, Kavakli, Ibrahim Halil, Zhang, Ray, Hayer, Katharina, Black, Michael B, Dueck, Hannah, Pizarro, Angel, Kim, Junhyong, Irizarry, Rafael, Thomas, Russell S, Grant, Gregory R, Hogenesch, John B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4197826/
https://www.ncbi.nlm.nih.gov/pubmed/24981968
http://dx.doi.org/10.1186/gb-2014-15-6-r86