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A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification

BACKGROUND: Porcine reproductive and respiratory syndrome virus (PRRSV), and particularly its highly pathogenic genotype (HP-PRRSV), have caused massive economic losses to the global swine industry. RESULTS: To rapidly identify HP-PRRSV, we developed a direct real-time reverse transcription polymera...

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Detalles Bibliográficos
Autores principales: Kang, Kang, Yang, Keli, Zhong, Jiasheng, Tian, Yongxiang, Zhang, Limin, Zhai, Jianxin, Zhang, Li, Song, Changxu, Gou, Christine Yuan, Luo, Jun, Gou, Deming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4198619/
https://www.ncbi.nlm.nih.gov/pubmed/25324970
http://dx.doi.org/10.1186/2049-1891-5-45