A Comparison between Droplet Digital and Quantitative PCR in the Analysis of Bacterial 16S Load in Lung Tissue Samples from Control and COPD GOLD 2

BACKGROUND: Low biomass in the bacterial lung tissue microbiome utilizes quantitative PCR (qPCR) 16S bacterial assays at their limit of detection. New technology like droplet digital PCR (ddPCR) could allow for higher sensitivity and accuracy of quantification. These attributes are needed if specifi...

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Detalles Bibliográficos
Autores principales: Sze, Marc A., Abbasi, Meysam, Hogg, James C., Sin, Don D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4199711/
https://www.ncbi.nlm.nih.gov/pubmed/25329701
http://dx.doi.org/10.1371/journal.pone.0110351

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4199711/
https://www.ncbi.nlm.nih.gov/pubmed/25329701
http://dx.doi.org/10.1371/journal.pone.0110351

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