In vitro and in vivo characterization of a West Nile virus MAD78 infectious clone

The viral determinants governing the varied neuropathogenicity of different West Nile virus (WNV) strains are poorly understood. Here, we generated an infectious clone (WNV-MAD(IC)) of the non-pathogenic strain WNV-MAD78 and compared its replication to that of parental WNV-MAD78 and a WNV-MAD78 infe...

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Autores principales: Hussmann, Katherine L., Vandergaast, Rianna, Ochsner, Susan Park, Huang, Albert C., Gale, Michael, Fredericksen, Brenda L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2014
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4200346/
https://www.ncbi.nlm.nih.gov/pubmed/25023336
http://dx.doi.org/10.1007/s00705-014-2176-2
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author Hussmann, Katherine L.
Vandergaast, Rianna
Ochsner, Susan Park
Huang, Albert C.
Gale, Michael
Fredericksen, Brenda L.
author_facet Hussmann, Katherine L.
Vandergaast, Rianna
Ochsner, Susan Park
Huang, Albert C.
Gale, Michael
Fredericksen, Brenda L.
author_sort Hussmann, Katherine L.
collection PubMed
description The viral determinants governing the varied neuropathogenicity of different West Nile virus (WNV) strains are poorly understood. Here, we generated an infectious clone (WNV-MAD(IC)) of the non-pathogenic strain WNV-MAD78 and compared its replication to that of parental WNV-MAD78 and a WNV-MAD78 infectious clone (WNV-MAD(TX-UTRs)) containing the 5′ and 3′ untranslated regions (UTRs) of the pathogenic strain WNV-TX. All three viruses replicated at similar rates and caused similar lethality in mice. Thus, the infectious clone is indistinguishable from parental virus in replication and neurovirulence, and the UTRs alone do not account for the increased virulence of WNV-TX compared to WNV-MAD78.
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spelling pubmed-42003462014-10-22 In vitro and in vivo characterization of a West Nile virus MAD78 infectious clone Hussmann, Katherine L. Vandergaast, Rianna Ochsner, Susan Park Huang, Albert C. Gale, Michael Fredericksen, Brenda L. Arch Virol Brief Report The viral determinants governing the varied neuropathogenicity of different West Nile virus (WNV) strains are poorly understood. Here, we generated an infectious clone (WNV-MAD(IC)) of the non-pathogenic strain WNV-MAD78 and compared its replication to that of parental WNV-MAD78 and a WNV-MAD78 infectious clone (WNV-MAD(TX-UTRs)) containing the 5′ and 3′ untranslated regions (UTRs) of the pathogenic strain WNV-TX. All three viruses replicated at similar rates and caused similar lethality in mice. Thus, the infectious clone is indistinguishable from parental virus in replication and neurovirulence, and the UTRs alone do not account for the increased virulence of WNV-TX compared to WNV-MAD78. Springer Vienna 2014-07-15 2014 /pmc/articles/PMC4200346/ /pubmed/25023336 http://dx.doi.org/10.1007/s00705-014-2176-2 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Brief Report
Hussmann, Katherine L.
Vandergaast, Rianna
Ochsner, Susan Park
Huang, Albert C.
Gale, Michael
Fredericksen, Brenda L.
In vitro and in vivo characterization of a West Nile virus MAD78 infectious clone
title In vitro and in vivo characterization of a West Nile virus MAD78 infectious clone
title_full In vitro and in vivo characterization of a West Nile virus MAD78 infectious clone
title_fullStr In vitro and in vivo characterization of a West Nile virus MAD78 infectious clone
title_full_unstemmed In vitro and in vivo characterization of a West Nile virus MAD78 infectious clone
title_short In vitro and in vivo characterization of a West Nile virus MAD78 infectious clone
title_sort in vitro and in vivo characterization of a west nile virus mad78 infectious clone
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4200346/
https://www.ncbi.nlm.nih.gov/pubmed/25023336
http://dx.doi.org/10.1007/s00705-014-2176-2
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