Expression of Aspergillus nidulans phy Gene in Nicotiana benthamiana Produces Active Phytase with Broad Specificities

A full-length phytase gene (phy) of Aspergillus nidulans was amplified from the cDNA library by polymerase chain reaction (PCR), and it was introduced into a bacterial expression vector, pET-28a. The recombinant protein (rPhy-E, 56 kDa) was overexpressed in the insoluble fraction of Escherichia coli...

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Detalles Bibliográficos
Autores principales: Oh, Tae-Kyun, Oh, Sung, Kim, Seongdae, Park, Jae Sung, Vinod, Nagarajan, Jang, Kyung Min, Kim, Sei Chang, Choi, Chang Won, Ko, Suk-Min, Jeong, Dong Kee, Udayakumar, Rajangam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4200759/
https://www.ncbi.nlm.nih.gov/pubmed/25192284
http://dx.doi.org/10.3390/ijms150915571

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4200759/
https://www.ncbi.nlm.nih.gov/pubmed/25192284
http://dx.doi.org/10.3390/ijms150915571

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