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Comprehensive Luciferase-Based Reporter Gene Assay Reveals Previously Masked Up-Regulatory Effects of miRNAs

MicroRNAs (miRNAs) are small non-coding RNAs that regulate the majority of the transcriptome at a post-transcriptional level. Because of this critical role, it is important to ensure that the assays used to determine their functionality are robust and reproducible. Typically, the reporter gene assay...

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Detalles Bibliográficos
Autores principales: Campos-Melo, Danae, Droppelmann, Cristian A., Volkening, Kathryn, Strong, Michael J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4200788/
https://www.ncbi.nlm.nih.gov/pubmed/25192285
http://dx.doi.org/10.3390/ijms150915592