High-throughput mutate-map-rescue evaluates SHAPE-directed RNA structure and uncovers excited states

The three-dimensional conformations of noncoding RNAs underpin their biochemical functions but have largely eluded experimental characterization. Here, we report that integrating a classic mutation/rescue strategy with high-throughput chemical mapping enables rapid RNA structure inference with unusu...

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Detalles Bibliográficos
Autores principales: Tian, Siqi, Cordero, Pablo, Kladwang, Wipapat, Das, Rhiju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2014
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4201832/
https://www.ncbi.nlm.nih.gov/pubmed/25183835
http://dx.doi.org/10.1261/rna.044321.114

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4201832/
https://www.ncbi.nlm.nih.gov/pubmed/25183835
http://dx.doi.org/10.1261/rna.044321.114

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