Cargando…
High-throughput mutate-map-rescue evaluates SHAPE-directed RNA structure and uncovers excited states
The three-dimensional conformations of noncoding RNAs underpin their biochemical functions but have largely eluded experimental characterization. Here, we report that integrating a classic mutation/rescue strategy with high-throughput chemical mapping enables rapid RNA structure inference with unusu...
| Autores principales: | , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Cold Spring Harbor Laboratory Press
2014
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4201832/ https://www.ncbi.nlm.nih.gov/pubmed/25183835 http://dx.doi.org/10.1261/rna.044321.114 |