Cargando…

Kinetic analysis of antagonist-occupied adenosine-A(3) receptors within membrane microdomains of individual cells provides evidence of receptor dimerization and allosterism

In our previous work, using a fluorescent adenosine-A(3) receptor (A(3)AR) agonist and fluorescence correlation spectroscopy (FCS), we demonstrated high-affinity labeling of the active receptor (R*) conformation. In the current study, we used a fluorescent A(3)AR antagonist (CA200645) to study the b...

Descripción completa

Detalles Bibliográficos
Autores principales:	Corriden, Ross, Kilpatrick, Laura E., Kellam, Barrie, Briddon, Stephen J., Hill, Stephen J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Federation of American Societies for Experimental Biology 2014
Materias:	Research Communications
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4202110/ https://www.ncbi.nlm.nih.gov/pubmed/24970394 http://dx.doi.org/10.1096/fj.13-247270

Ejemplares similares

Adenosine-A(3) receptors in neutrophil microdomains promote the formation of bacteria-tethering cytonemes
por: Corriden, Ross, et al.
Publicado: (2013)

Efficient G protein coupling is not required for agonist‐mediated internalization and membrane reorganization of the adenosine A(3) receptor
por: Stoddart, Leigh A., et al.
Publicado: (2021)

NanoBiT Complementation to Monitor Agonist-Induced Adenosine A(1) Receptor Internalization
por: Soave, Mark, et al.
Publicado: (2019)

Effect of a toggle switch mutation in TM6 of the human adenosine A(3) receptor on Gi protein-dependent signalling and Gi-independent receptor internalization
por: Stoddart, Leigh A, et al.
Publicado: (2014)

Influence of fluorophore and linker composition on the pharmacology of fluorescent adenosine A(1) receptor ligands
por: Baker, Jillian G, et al.
Publicado: (2010)

Allosteric interactions across native adenosine-A(3) receptor homodimers: quantification using single-cell ligand-binding kinetics
por: May, Lauren T., et al.
Publicado: (2011)

Fragment Screening at Adenosine-A(3) Receptors in Living Cells Using a Fluorescence-Based Binding Assay
por: Stoddart, Leigh A., et al.
Publicado: (2012)

A Non-imaging High Throughput Approach to Chemical Library Screening at the Unmodified Adenosine-A(3) Receptor in Living Cells
por: Arruda, Maria Augusta, et al.
Publicado: (2017)

Development of novel fluorescent histamine H(1)-receptor antagonists to study ligand-binding kinetics in living cells
por: Stoddart, Leigh A., et al.
Publicado: (2018)

Monitoring Allosteric Interactions with CXCR4 Using NanoBiT Conjugated Nanobodies
por: Soave, Mark, et al.
Publicado: (2020)

Allosteric modulation of adenosine receptors
por: Göblyös, Anikó, et al.
Publicado: (2008)

Small-Molecule Fluorescent Ligands for the CXCR4 Chemokine Receptor
por: Dekkers, Sebastian, et al.
Publicado: (2023)

A live cell NanoBRET binding assay allows the study of ligand-binding kinetics to the adenosine A(3) receptor
por: Bouzo-Lorenzo, Monica, et al.
Publicado: (2019)

Allosteric antagonist action at triheteromeric NMDA receptors
por: Gibb, Alasdair J.
Publicado: (2022)

Allosteric interactions prime androgen receptor dimerization and activation
por: Wasmuth, Elizabeth V., et al.
Publicado: (2022)

Allosteric modulation of the adenosine A(2A) receptor by cholesterol
por: Huang, Shuya Kate, et al.
Publicado: (2022)

Small molecule allosteric modulation of the adenosine A(1) receptor
por: Nguyen, Anh T. N., et al.
Publicado: (2023)

Synthesis and Evaluation of the First Fluorescent Antagonists of the Human P2Y(2) Receptor Based on AR-C118925
por: Conroy, Sean, et al.
Publicado: (2018)

Correction to Synthesis and Evaluation of the First Fluorescent Antagonists of the Human P2Y(2) Receptor Based on AR-C118925
por: Conroy, Sean, et al.
Publicado: (2018)

Update on the recent development of allosteric modulators for adenosine receptors and their therapeutic applications
por: Pasquini, Silvia, et al.
Publicado: (2022)

Kinetic profiling and functional characterization of 8-phenylxanthine derivatives as A(2B) adenosine receptor antagonists
por: Vlachodimou, Anna, et al.
Publicado: (2022)

Probe dependence of allosteric enhancers on the binding affinity of adenosine A(1)‐receptor agonists at rat and human A(1)‐receptors measured using NanoBRET
por: Cooper, Samantha L., et al.
Publicado: (2019)

Structural Characterization of Receptor–Receptor Interactions in the Allosteric Modulation of G Protein-Coupled Receptor (GPCR) Dimers
por: Lazim, Raudah, et al.
Publicado: (2021)

Irreversible Antagonists for the Adenosine A(2B) Receptor †
por: Temirak, Ahmed, et al.
Publicado: (2022)

Role of Stoichiometry in the Dimer-Stabilizing Effect of AMPA Receptor Allosteric Modulators
por: Ptak, Christopher P., et al.
Publicado: (2013)

Structure of CC Chemokine Receptor 2 with Orthosteric and Allosteric Antagonists
por: Zheng, Yi, et al.
Publicado: (2016)

Positive allosteric modulation of the adenosine A(2a) receptor attenuates inflammation
por: Welihinda, Ajith A, et al.
Publicado: (2014)

Structural Basis for Binding of Allosteric Drug Leads in the Adenosine A(1) Receptor
por: Miao, Yinglong, et al.
Publicado: (2018)

Dimerization with Cannabinoid Receptors Allosterically Modulates Delta Opioid Receptor Activity during Neuropathic Pain
por: Bushlin, Ittai, et al.
Publicado: (2012)

Effect of Adenosine Receptor Antagonists on Adenosine-Pretreated PC12 Cells Exposed to Paraquat
por: Shang, Liangcheng, et al.
Publicado: (2022)

Internalization of the chemokine receptor CCR4 can be evoked by orthosteric and allosteric receptor antagonists
por: Ajram, Laura, et al.
Publicado: (2014)

Identification of Novel Adenosine A(2A) Receptor Antagonists by Virtual Screening
por: Langmead, Christopher J., et al.
Publicado: (2012)

Novel selective antagonist radioligands for the pharmacological study of A(2B) adenosine receptors
por: Gessi, Stefania, et al.
Publicado: (2006)

The glucocorticoid receptor dimer interface allosterically transmits sequence-specific DNA signals
por: Watson, Lisa C., et al.
Publicado: (2013)

Pharmacological characterisation of novel adenosine A(3) receptor antagonists
por: Barkan, Kerry, et al.
Publicado: (2020)

Visualizing Cyclic Adenosine Monophosphate in Cardiac Microdomains Involved in Ion Homeostasis
por: Dikolayev, Vladimir, et al.
Publicado: (2019)

Detection of genome‐edited and endogenously expressed G protein‐coupled receptors
por: Soave, Mark, et al.
Publicado: (2021)

Quantitative analysis of neuropeptide Y receptor association with β-arrestin2 measured by bimolecular fluorescence complementation
por: Kilpatrick, LE, et al.
Publicado: (2010)

Pharmacology under the microscope: the use of fluorescence correlation spectroscopy to determine the properties of ligand–receptor complexes
por: Briddon, Stephen J., et al.
Publicado: (2007)

Allosteric Antagonism of the A(2A) Adenosine Receptor by a Series of Bitopic Ligands
por: Gao, Zhan-Guo, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_s3hl2ho1puqhs7islv7gcbgnct