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Single-stranded positive-sense RNA viruses generated in days using infectious subgenomic amplicons

Reverse genetics is a key methodology for producing genetically modified RNA viruses and deciphering cellular and viral biological properties, but methods based on the preparation of plasmid-based complete viral genomes are laborious and unpredictable. Here, both wild-type and genetically modified i...

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Detalles Bibliográficos
Autores principales: Aubry, Fabien, Nougairède, Antoine, de Fabritus, Lauriane, Querat, Gilles, Gould, Ernest A., de Lamballerie, Xavier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society for General Microbiology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4202267/
https://www.ncbi.nlm.nih.gov/pubmed/25053561
http://dx.doi.org/10.1099/vir.0.068023-0