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Single-stranded positive-sense RNA viruses generated in days using infectious subgenomic amplicons
Reverse genetics is a key methodology for producing genetically modified RNA viruses and deciphering cellular and viral biological properties, but methods based on the preparation of plasmid-based complete viral genomes are laborious and unpredictable. Here, both wild-type and genetically modified i...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society for General Microbiology
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4202267/ https://www.ncbi.nlm.nih.gov/pubmed/25053561 http://dx.doi.org/10.1099/vir.0.068023-0 |