Single-stranded positive-sense RNA viruses generated in days using infectious subgenomic amplicons

Reverse genetics is a key methodology for producing genetically modified RNA viruses and deciphering cellular and viral biological properties, but methods based on the preparation of plasmid-based complete viral genomes are laborious and unpredictable. Here, both wild-type and genetically modified i...

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Autores principales: Aubry, Fabien, Nougairède, Antoine, de Fabritus, Lauriane, Querat, Gilles, Gould, Ernest A., de Lamballerie, Xavier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society for General Microbiology 2014
Materias:
Animal
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4202267/
https://www.ncbi.nlm.nih.gov/pubmed/25053561
http://dx.doi.org/10.1099/vir.0.068023-0
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author Aubry, Fabien
Nougairède, Antoine
de Fabritus, Lauriane
Querat, Gilles
Gould, Ernest A.
de Lamballerie, Xavier
author_facet Aubry, Fabien
Nougairède, Antoine
de Fabritus, Lauriane
Querat, Gilles
Gould, Ernest A.
de Lamballerie, Xavier
author_sort Aubry, Fabien
collection PubMed
description Reverse genetics is a key methodology for producing genetically modified RNA viruses and deciphering cellular and viral biological properties, but methods based on the preparation of plasmid-based complete viral genomes are laborious and unpredictable. Here, both wild-type and genetically modified infectious RNA viruses were generated in days using the newly described ISA (infectious-subgenomic-amplicons) method. This new versatile and simple procedure may enhance our capacity to obtain infectious RNA viruses from PCR-amplified genetic material.
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spelling pubmed-42022672014-11-04 Single-stranded positive-sense RNA viruses generated in days using infectious subgenomic amplicons Aubry, Fabien Nougairède, Antoine de Fabritus, Lauriane Querat, Gilles Gould, Ernest A. de Lamballerie, Xavier J Gen Virol Animal Reverse genetics is a key methodology for producing genetically modified RNA viruses and deciphering cellular and viral biological properties, but methods based on the preparation of plasmid-based complete viral genomes are laborious and unpredictable. Here, both wild-type and genetically modified infectious RNA viruses were generated in days using the newly described ISA (infectious-subgenomic-amplicons) method. This new versatile and simple procedure may enhance our capacity to obtain infectious RNA viruses from PCR-amplified genetic material. Society for General Microbiology 2014-11 /pmc/articles/PMC4202267/ /pubmed/25053561 http://dx.doi.org/10.1099/vir.0.068023-0 Text en © 2014 The Authors http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Animal
Aubry, Fabien
Nougairède, Antoine
de Fabritus, Lauriane
Querat, Gilles
Gould, Ernest A.
de Lamballerie, Xavier
Single-stranded positive-sense RNA viruses generated in days using infectious subgenomic amplicons
title Single-stranded positive-sense RNA viruses generated in days using infectious subgenomic amplicons
title_full Single-stranded positive-sense RNA viruses generated in days using infectious subgenomic amplicons
title_fullStr Single-stranded positive-sense RNA viruses generated in days using infectious subgenomic amplicons
title_full_unstemmed Single-stranded positive-sense RNA viruses generated in days using infectious subgenomic amplicons
title_short Single-stranded positive-sense RNA viruses generated in days using infectious subgenomic amplicons
title_sort single-stranded positive-sense rna viruses generated in days using infectious subgenomic amplicons
topic Animal
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4202267/
https://www.ncbi.nlm.nih.gov/pubmed/25053561
http://dx.doi.org/10.1099/vir.0.068023-0
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