Sorting cells for basal and induced autophagic flux by quantitative ratiometric flow cytometry

We detail here a protocol using tandem-tagged mCherry-EGFP-LC3 (C-G-LC3) to quantify autophagic flux in single cells by ratiometric flow cytometry and to isolate subpopulations of cells based on their relative levels of autophagic flux. This robust and sensitive method measures autophagic flux rathe...

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Detalles Bibliográficos
Autores principales: Gump, Jacob M, Thorburn, Andrew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2014
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4203556/
https://www.ncbi.nlm.nih.gov/pubmed/24915460
http://dx.doi.org/10.4161/auto.29394

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4203556/
https://www.ncbi.nlm.nih.gov/pubmed/24915460
http://dx.doi.org/10.4161/auto.29394

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