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Sorting cells for basal and induced autophagic flux by quantitative ratiometric flow cytometry
We detail here a protocol using tandem-tagged mCherry-EGFP-LC3 (C-G-LC3) to quantify autophagic flux in single cells by ratiometric flow cytometry and to isolate subpopulations of cells based on their relative levels of autophagic flux. This robust and sensitive method measures autophagic flux rathe...
Autores principales: | Gump, Jacob M, Thorburn, Andrew |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Landes Bioscience
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4203556/ https://www.ncbi.nlm.nih.gov/pubmed/24915460 http://dx.doi.org/10.4161/auto.29394 |
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