The Impact of HIF1α on the Per2 Circadian Rhythm in Renal Cancer Cell Lines

In mammals, the circadian rhythm central generator consists of interactions among clock genes, including Per1/2/3, Cry1/2, Bmal1, and Clock. Circadian rhythm disruption may lead to increased risk of cancer in humans, and deregulation of clock genes has been implicated in many types of cancers. Among...

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Autores principales: Okabe, Takashi, Kumagai, Megumi, Nakajima, Yoshihiro, Shirotake, Suguru, Kodaira, Kiichiro, Oyama, Masafumi, Ueno, Munehisa, Ikeda, Masaaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204850/
https://www.ncbi.nlm.nih.gov/pubmed/25333958
http://dx.doi.org/10.1371/journal.pone.0109693
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author Okabe, Takashi
Kumagai, Megumi
Nakajima, Yoshihiro
Shirotake, Suguru
Kodaira, Kiichiro
Oyama, Masafumi
Ueno, Munehisa
Ikeda, Masaaki
author_facet Okabe, Takashi
Kumagai, Megumi
Nakajima, Yoshihiro
Shirotake, Suguru
Kodaira, Kiichiro
Oyama, Masafumi
Ueno, Munehisa
Ikeda, Masaaki
author_sort Okabe, Takashi
collection PubMed
description In mammals, the circadian rhythm central generator consists of interactions among clock genes, including Per1/2/3, Cry1/2, Bmal1, and Clock. Circadian rhythm disruption may lead to increased risk of cancer in humans, and deregulation of clock genes has been implicated in many types of cancers. Among these genes, Per2 is reported to have tumor suppressor properties, but little is known about the correlation between Per2 and HIF, which is the main target of renal cell carcinoma (RCC) therapy. In this study, the rhythmic expression of the Per2 gene was not detectable in renal cancer cell lines, with the exception of Caki-2 cells. In Caki-2 cells, HIF1α increased the amplitude of Per2 oscillation by directly binding to the HIF-binding site located on the Per2 promoter. These results indicate that HIF1α may enhance the amplitude of the Per2 circadian rhythm.
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spelling pubmed-42048502014-10-27 The Impact of HIF1α on the Per2 Circadian Rhythm in Renal Cancer Cell Lines Okabe, Takashi Kumagai, Megumi Nakajima, Yoshihiro Shirotake, Suguru Kodaira, Kiichiro Oyama, Masafumi Ueno, Munehisa Ikeda, Masaaki PLoS One Research Article In mammals, the circadian rhythm central generator consists of interactions among clock genes, including Per1/2/3, Cry1/2, Bmal1, and Clock. Circadian rhythm disruption may lead to increased risk of cancer in humans, and deregulation of clock genes has been implicated in many types of cancers. Among these genes, Per2 is reported to have tumor suppressor properties, but little is known about the correlation between Per2 and HIF, which is the main target of renal cell carcinoma (RCC) therapy. In this study, the rhythmic expression of the Per2 gene was not detectable in renal cancer cell lines, with the exception of Caki-2 cells. In Caki-2 cells, HIF1α increased the amplitude of Per2 oscillation by directly binding to the HIF-binding site located on the Per2 promoter. These results indicate that HIF1α may enhance the amplitude of the Per2 circadian rhythm. Public Library of Science 2014-10-21 /pmc/articles/PMC4204850/ /pubmed/25333958 http://dx.doi.org/10.1371/journal.pone.0109693 Text en © 2014 Okabe et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Okabe, Takashi
Kumagai, Megumi
Nakajima, Yoshihiro
Shirotake, Suguru
Kodaira, Kiichiro
Oyama, Masafumi
Ueno, Munehisa
Ikeda, Masaaki
The Impact of HIF1α on the Per2 Circadian Rhythm in Renal Cancer Cell Lines
title The Impact of HIF1α on the Per2 Circadian Rhythm in Renal Cancer Cell Lines
title_full The Impact of HIF1α on the Per2 Circadian Rhythm in Renal Cancer Cell Lines
title_fullStr The Impact of HIF1α on the Per2 Circadian Rhythm in Renal Cancer Cell Lines
title_full_unstemmed The Impact of HIF1α on the Per2 Circadian Rhythm in Renal Cancer Cell Lines
title_short The Impact of HIF1α on the Per2 Circadian Rhythm in Renal Cancer Cell Lines
title_sort impact of hif1α on the per2 circadian rhythm in renal cancer cell lines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204850/
https://www.ncbi.nlm.nih.gov/pubmed/25333958
http://dx.doi.org/10.1371/journal.pone.0109693
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