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The Bioconversion of Red Ginseng Ethanol Extract into Compound K by Saccharomyces cerevisiae HJ-014
A β-glucosidase producing yeast strain was isolated from Korean traditional rice wine. Based on the sequence of the YCL008c gene and analysis of the fatty acid composition, the isolate was identified as Saccharomyces cerevisiae strain HJ-014. S. cerevisiae HJ-014 produced ginsenoside Rd, F(2), and c...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Mycology
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4206791/ https://www.ncbi.nlm.nih.gov/pubmed/25346602 http://dx.doi.org/10.5941/MYCO.2014.42.3.256 |
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author | Choi, Hak Joo Kim, Eun A Kim, Dong Hee Shin, Kwang-Soo |
author_facet | Choi, Hak Joo Kim, Eun A Kim, Dong Hee Shin, Kwang-Soo |
author_sort | Choi, Hak Joo |
collection | PubMed |
description | A β-glucosidase producing yeast strain was isolated from Korean traditional rice wine. Based on the sequence of the YCL008c gene and analysis of the fatty acid composition, the isolate was identified as Saccharomyces cerevisiae strain HJ-014. S. cerevisiae HJ-014 produced ginsenoside Rd, F(2), and compound K from the ethanol extract of red ginseng. The production was increased by shaking culture, where the bioconversion efficiency was increased 2-fold compared to standing culture. The production of ginsenoside F(2) and compound K was time-dependent and thought to proceed by the transformation pathway of: red ginseng extract→Rd→F(2)→compound K. The optimum incubation time and concentration of red ginseng extract for the production of compound K was 96 hr and 4.5% (w/v), respectively. |
format | Online Article Text |
id | pubmed-4206791 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | The Korean Society of Mycology |
record_format | MEDLINE/PubMed |
spelling | pubmed-42067912014-10-24 The Bioconversion of Red Ginseng Ethanol Extract into Compound K by Saccharomyces cerevisiae HJ-014 Choi, Hak Joo Kim, Eun A Kim, Dong Hee Shin, Kwang-Soo Mycobiology Research Article A β-glucosidase producing yeast strain was isolated from Korean traditional rice wine. Based on the sequence of the YCL008c gene and analysis of the fatty acid composition, the isolate was identified as Saccharomyces cerevisiae strain HJ-014. S. cerevisiae HJ-014 produced ginsenoside Rd, F(2), and compound K from the ethanol extract of red ginseng. The production was increased by shaking culture, where the bioconversion efficiency was increased 2-fold compared to standing culture. The production of ginsenoside F(2) and compound K was time-dependent and thought to proceed by the transformation pathway of: red ginseng extract→Rd→F(2)→compound K. The optimum incubation time and concentration of red ginseng extract for the production of compound K was 96 hr and 4.5% (w/v), respectively. The Korean Society of Mycology 2014-09 2014-09-30 /pmc/articles/PMC4206791/ /pubmed/25346602 http://dx.doi.org/10.5941/MYCO.2014.42.3.256 Text en © The Korean Society of Mycology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Choi, Hak Joo Kim, Eun A Kim, Dong Hee Shin, Kwang-Soo The Bioconversion of Red Ginseng Ethanol Extract into Compound K by Saccharomyces cerevisiae HJ-014 |
title | The Bioconversion of Red Ginseng Ethanol Extract into Compound K by Saccharomyces cerevisiae HJ-014 |
title_full | The Bioconversion of Red Ginseng Ethanol Extract into Compound K by Saccharomyces cerevisiae HJ-014 |
title_fullStr | The Bioconversion of Red Ginseng Ethanol Extract into Compound K by Saccharomyces cerevisiae HJ-014 |
title_full_unstemmed | The Bioconversion of Red Ginseng Ethanol Extract into Compound K by Saccharomyces cerevisiae HJ-014 |
title_short | The Bioconversion of Red Ginseng Ethanol Extract into Compound K by Saccharomyces cerevisiae HJ-014 |
title_sort | bioconversion of red ginseng ethanol extract into compound k by saccharomyces cerevisiae hj-014 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4206791/ https://www.ncbi.nlm.nih.gov/pubmed/25346602 http://dx.doi.org/10.5941/MYCO.2014.42.3.256 |
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