In vitro nanobody discovery for integral membrane protein targets

Nanobodies (Nbs) or single-domain antibodies are among the smallest and most stable binder scaffolds known. In vitro display is a powerful antibody discovery technique used worldwide. We describe the first adaptation of in vitro mRNA/cDNA display for the rapid, automatable discovery of Nbs against d...

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Autores principales: Doshi, Rupak, Chen, Beverly R., Vibat, Cecile Rose T., Huang, Norman, Lee, Chang-Wook, Chang, Geoffrey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4208029/
https://www.ncbi.nlm.nih.gov/pubmed/25342225
http://dx.doi.org/10.1038/srep06760
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author Doshi, Rupak
Chen, Beverly R.
Vibat, Cecile Rose T.
Huang, Norman
Lee, Chang-Wook
Chang, Geoffrey
author_facet Doshi, Rupak
Chen, Beverly R.
Vibat, Cecile Rose T.
Huang, Norman
Lee, Chang-Wook
Chang, Geoffrey
author_sort Doshi, Rupak
collection PubMed
description Nanobodies (Nbs) or single-domain antibodies are among the smallest and most stable binder scaffolds known. In vitro display is a powerful antibody discovery technique used worldwide. We describe the first adaptation of in vitro mRNA/cDNA display for the rapid, automatable discovery of Nbs against desired targets, and use it to discover the first ever reported nanobody against the human full-length glucose transporter, GLUT-1. We envision our streamlined method as a bench-top platform technology, in combination with various molecular evolution techniques, for expedited Nb discovery.
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spelling pubmed-42080292014-10-27 In vitro nanobody discovery for integral membrane protein targets Doshi, Rupak Chen, Beverly R. Vibat, Cecile Rose T. Huang, Norman Lee, Chang-Wook Chang, Geoffrey Sci Rep Article Nanobodies (Nbs) or single-domain antibodies are among the smallest and most stable binder scaffolds known. In vitro display is a powerful antibody discovery technique used worldwide. We describe the first adaptation of in vitro mRNA/cDNA display for the rapid, automatable discovery of Nbs against desired targets, and use it to discover the first ever reported nanobody against the human full-length glucose transporter, GLUT-1. We envision our streamlined method as a bench-top platform technology, in combination with various molecular evolution techniques, for expedited Nb discovery. Nature Publishing Group 2014-10-24 /pmc/articles/PMC4208029/ /pubmed/25342225 http://dx.doi.org/10.1038/srep06760 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Article
Doshi, Rupak
Chen, Beverly R.
Vibat, Cecile Rose T.
Huang, Norman
Lee, Chang-Wook
Chang, Geoffrey
In vitro nanobody discovery for integral membrane protein targets
title In vitro nanobody discovery for integral membrane protein targets
title_full In vitro nanobody discovery for integral membrane protein targets
title_fullStr In vitro nanobody discovery for integral membrane protein targets
title_full_unstemmed In vitro nanobody discovery for integral membrane protein targets
title_short In vitro nanobody discovery for integral membrane protein targets
title_sort in vitro nanobody discovery for integral membrane protein targets
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4208029/
https://www.ncbi.nlm.nih.gov/pubmed/25342225
http://dx.doi.org/10.1038/srep06760
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