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Recognition of Chelerythrine to Human Telomeric DNA and RNA G-quadruplexes

A study on binding of antitumor chelerythrine to human telomeric DNA/RNA G-quadruplexes was performed by using DNA polymerase stop assay, UV-melting, ESI-TOF-MS, UV-Vis absorption spectrophotometry and fluorescent triazole orange displacement assay. Chelerythrine selectively binds to and stabilizes...

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Autores principales: Bai, Li-Ping, Hagihara, Masaki, Nakatani, Kazuhiko, Jiang, Zhi-Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4208030/
https://www.ncbi.nlm.nih.gov/pubmed/25341562
http://dx.doi.org/10.1038/srep06767
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author Bai, Li-Ping
Hagihara, Masaki
Nakatani, Kazuhiko
Jiang, Zhi-Hong
author_facet Bai, Li-Ping
Hagihara, Masaki
Nakatani, Kazuhiko
Jiang, Zhi-Hong
author_sort Bai, Li-Ping
collection PubMed
description A study on binding of antitumor chelerythrine to human telomeric DNA/RNA G-quadruplexes was performed by using DNA polymerase stop assay, UV-melting, ESI-TOF-MS, UV-Vis absorption spectrophotometry and fluorescent triazole orange displacement assay. Chelerythrine selectively binds to and stabilizes the K(+)-form hybrid-type human telomeric DNA G-quadruplex of biological significance, compared with the Na(+)-form antiparallel-type DNA G-quadruplex. ESI-TOF-MS study showed that chelerythrine possesses a binding strength for DNA G-quadruplex comparable to that of TMPyP4 tetrachloride. Both 1:1 and 2:1 stoichiometries were observed for chelerythrine's binding with DNA and RNA G-quadruplexes. The binding strength of chelerythrine with RNA G-quadruplex is stronger than that with DNA G-quadruplex. Fluorescent triazole orange displacement assay revealed that chelerythrine interacts with human telomeric RNA/DNA G-quadruplexes by the mode of end- stacking. The relative binding strength of chelerythrine for human telomeric RNA and DNA G-quadruplexes obtained from ESI-TOF-MS experiments are respectively 6.0- and 2.5-fold tighter than that with human telomeric double-stranded hairpin DNA. The binding selectivity of chelerythrine for the biologically significant K(+)-form human telomeric DNA G-quadruplex over the Na(+)-form analogue, and binding specificity for human telomeric RNA G-quadruplex established it as a promising candidate in the structure-based design and development of G-quadruplex specific ligands.
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spelling pubmed-42080302014-10-27 Recognition of Chelerythrine to Human Telomeric DNA and RNA G-quadruplexes Bai, Li-Ping Hagihara, Masaki Nakatani, Kazuhiko Jiang, Zhi-Hong Sci Rep Article A study on binding of antitumor chelerythrine to human telomeric DNA/RNA G-quadruplexes was performed by using DNA polymerase stop assay, UV-melting, ESI-TOF-MS, UV-Vis absorption spectrophotometry and fluorescent triazole orange displacement assay. Chelerythrine selectively binds to and stabilizes the K(+)-form hybrid-type human telomeric DNA G-quadruplex of biological significance, compared with the Na(+)-form antiparallel-type DNA G-quadruplex. ESI-TOF-MS study showed that chelerythrine possesses a binding strength for DNA G-quadruplex comparable to that of TMPyP4 tetrachloride. Both 1:1 and 2:1 stoichiometries were observed for chelerythrine's binding with DNA and RNA G-quadruplexes. The binding strength of chelerythrine with RNA G-quadruplex is stronger than that with DNA G-quadruplex. Fluorescent triazole orange displacement assay revealed that chelerythrine interacts with human telomeric RNA/DNA G-quadruplexes by the mode of end- stacking. The relative binding strength of chelerythrine for human telomeric RNA and DNA G-quadruplexes obtained from ESI-TOF-MS experiments are respectively 6.0- and 2.5-fold tighter than that with human telomeric double-stranded hairpin DNA. The binding selectivity of chelerythrine for the biologically significant K(+)-form human telomeric DNA G-quadruplex over the Na(+)-form analogue, and binding specificity for human telomeric RNA G-quadruplex established it as a promising candidate in the structure-based design and development of G-quadruplex specific ligands. Nature Publishing Group 2014-10-24 /pmc/articles/PMC4208030/ /pubmed/25341562 http://dx.doi.org/10.1038/srep06767 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
spellingShingle Article
Bai, Li-Ping
Hagihara, Masaki
Nakatani, Kazuhiko
Jiang, Zhi-Hong
Recognition of Chelerythrine to Human Telomeric DNA and RNA G-quadruplexes
title Recognition of Chelerythrine to Human Telomeric DNA and RNA G-quadruplexes
title_full Recognition of Chelerythrine to Human Telomeric DNA and RNA G-quadruplexes
title_fullStr Recognition of Chelerythrine to Human Telomeric DNA and RNA G-quadruplexes
title_full_unstemmed Recognition of Chelerythrine to Human Telomeric DNA and RNA G-quadruplexes
title_short Recognition of Chelerythrine to Human Telomeric DNA and RNA G-quadruplexes
title_sort recognition of chelerythrine to human telomeric dna and rna g-quadruplexes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4208030/
https://www.ncbi.nlm.nih.gov/pubmed/25341562
http://dx.doi.org/10.1038/srep06767
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