Reversal of Anchorage-Independent Multicellular Spheroid into a Monolayer Mimics a Metastatic Model

Lack of an in vitro model of metastasis has been a major impediment in understanding the molecular regulation of metastatic processes, and identification of specific therapeutic targets. We have established an in vitro model which displayed the signatures of metastatic phenotype such as migration, i...

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Autores principales: Kunjithapatham, Rani, Karthikeyan, Swathi, Geschwind, Jean-Francois, Kieserman, Esther, Lin, MingDe, Fu, De-Xue, Ganapathy-Kanniappan, Shanmugasundaram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4212233/
https://www.ncbi.nlm.nih.gov/pubmed/25351825
http://dx.doi.org/10.1038/srep06816
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author Kunjithapatham, Rani
Karthikeyan, Swathi
Geschwind, Jean-Francois
Kieserman, Esther
Lin, MingDe
Fu, De-Xue
Ganapathy-Kanniappan, Shanmugasundaram
author_facet Kunjithapatham, Rani
Karthikeyan, Swathi
Geschwind, Jean-Francois
Kieserman, Esther
Lin, MingDe
Fu, De-Xue
Ganapathy-Kanniappan, Shanmugasundaram
author_sort Kunjithapatham, Rani
collection PubMed
description Lack of an in vitro model of metastasis has been a major impediment in understanding the molecular regulation of metastatic processes, and identification of specific therapeutic targets. We have established an in vitro model which displayed the signatures of metastatic phenotype such as migration, invasiveness, chemoresistance and expression of cancer stem-cell markers. This in vitro model was developed by the induction of reversal of multicellular spheroids that were generated by anchorage-independent growth. In vivo data further validated the metastatic phenotype of the in vitro model. Besides delineating the molecular events of metastasis, this model could also improve the screening efficiency of antimetastatic agents.
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spelling pubmed-42122332014-10-31 Reversal of Anchorage-Independent Multicellular Spheroid into a Monolayer Mimics a Metastatic Model Kunjithapatham, Rani Karthikeyan, Swathi Geschwind, Jean-Francois Kieserman, Esther Lin, MingDe Fu, De-Xue Ganapathy-Kanniappan, Shanmugasundaram Sci Rep Article Lack of an in vitro model of metastasis has been a major impediment in understanding the molecular regulation of metastatic processes, and identification of specific therapeutic targets. We have established an in vitro model which displayed the signatures of metastatic phenotype such as migration, invasiveness, chemoresistance and expression of cancer stem-cell markers. This in vitro model was developed by the induction of reversal of multicellular spheroids that were generated by anchorage-independent growth. In vivo data further validated the metastatic phenotype of the in vitro model. Besides delineating the molecular events of metastasis, this model could also improve the screening efficiency of antimetastatic agents. Nature Publishing Group 2014-10-29 /pmc/articles/PMC4212233/ /pubmed/25351825 http://dx.doi.org/10.1038/srep06816 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Article
Kunjithapatham, Rani
Karthikeyan, Swathi
Geschwind, Jean-Francois
Kieserman, Esther
Lin, MingDe
Fu, De-Xue
Ganapathy-Kanniappan, Shanmugasundaram
Reversal of Anchorage-Independent Multicellular Spheroid into a Monolayer Mimics a Metastatic Model
title Reversal of Anchorage-Independent Multicellular Spheroid into a Monolayer Mimics a Metastatic Model
title_full Reversal of Anchorage-Independent Multicellular Spheroid into a Monolayer Mimics a Metastatic Model
title_fullStr Reversal of Anchorage-Independent Multicellular Spheroid into a Monolayer Mimics a Metastatic Model
title_full_unstemmed Reversal of Anchorage-Independent Multicellular Spheroid into a Monolayer Mimics a Metastatic Model
title_short Reversal of Anchorage-Independent Multicellular Spheroid into a Monolayer Mimics a Metastatic Model
title_sort reversal of anchorage-independent multicellular spheroid into a monolayer mimics a metastatic model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4212233/
https://www.ncbi.nlm.nih.gov/pubmed/25351825
http://dx.doi.org/10.1038/srep06816
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