Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics

Quantitative proteomics combined with immuno-affinity purification, SILAC immunoprecipitation, represent a powerful means for the discovery of novel protein:protein interactions. By allowing the accurate relative quantification of protein abundance in both control and test samples, true interactions...

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Detalles Bibliográficos
Autores principales: Emmott, Edward, Goodfellow, Ian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2014
Materias:
Biochemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4212580/
https://www.ncbi.nlm.nih.gov/pubmed/25046639
http://dx.doi.org/10.3791/51656

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4212580/
https://www.ncbi.nlm.nih.gov/pubmed/25046639
http://dx.doi.org/10.3791/51656

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