Effect of RNA integrity on uniquely mapped reads in RNA-Seq

BACKGROUND: We examined the performance of three RNA-Sequencing library preparation protocols as a function of RNA integrity, comparing gene expressions between heat-degraded samples to their high-quality counterparts. This work is invaluable given the difficulty of obtaining high-quality RNA from t...

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Detalles Bibliográficos
Autores principales: Chen, Emily A, Souaiaia, Tade, Herstein, Jennifer S, Evgrafov, Oleg V, Spitsyna, Valeria N, Rebolini, Danea F, Knowles, James A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Correspondence
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4213542/
https://www.ncbi.nlm.nih.gov/pubmed/25339126
http://dx.doi.org/10.1186/1756-0500-7-753

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4213542/
https://www.ncbi.nlm.nih.gov/pubmed/25339126
http://dx.doi.org/10.1186/1756-0500-7-753

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