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Effect of RNA integrity on uniquely mapped reads in RNA-Seq

BACKGROUND: We examined the performance of three RNA-Sequencing library preparation protocols as a function of RNA integrity, comparing gene expressions between heat-degraded samples to their high-quality counterparts. This work is invaluable given the difficulty of obtaining high-quality RNA from t...

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Detalles Bibliográficos
Autores principales:	Chen, Emily A, Souaiaia, Tade, Herstein, Jennifer S, Evgrafov, Oleg V, Spitsyna, Valeria N, Rebolini, Danea F, Knowles, James A
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2014
Materias:	Correspondence
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4213542/ https://www.ncbi.nlm.nih.gov/pubmed/25339126 http://dx.doi.org/10.1186/1756-0500-7-753

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